AB130456

FITC Anti-CD99 antibody [3B2/TA8]

Name: Anti-CD99 FITC antibody [3B2/TA8] (ab130456) Mouse monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB130456
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD99 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD99 protein.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

2 Images

Flow Cytometry - FITC Anti-CD99 antibody [3B2/TA8] (AB130456)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD99 antibody [3B2/TA8] (AB130456)

Flow cytometry analysis (surface staining) of human peripheral whole blood labeling CD99 with ab130456 (4 µL reagent/100 µL of peripheral whole blood). Histogram showing separation of human CD99 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD99 antibody [3B2/TA8] (AB130456)

Flow cytometry surface staining pattern of human peripheral whole blood labeling CD99 with ab130456 (4 µL reagent/100 µL of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3B2/TA8

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD99 protein. The exact immunogen used to generate this antibody is proprietary information.

P14209

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.

Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

See full target information CD99

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 75:7175-83 PubMed11435598

2001

Lipid rafts and pseudotyping.

Applications

Unspecified application

Species

Unspecified reactive species

W F Pickl,F X Pimentel-Muiños,B Seed

Journal of immunology (Baltimore, Md. : 1950) 161:4671-8 PubMed9794396

1998

CD99 engagement on human peripheral blood T cells results in TCR/CD3-dependent cellular activation and allows for Th1-restricted cytokine production.

Applications

Unspecified application

Species

Unspecified reactive species

M Waclavicek,O Majdic,T Stulnig,M Berger,R Sunder-Plassmann,G J Zlabinger,T Baumruker,J Stöckl,C Ebner,W Knapp,W F Pickl

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-CD99 antibody [3B2/TA8]

Flow Cytometry - FITC Anti-CD99 antibody [3B2/TA8] (AB130456)

Flow Cytometry - FITC Anti-CD99 antibody [3B2/TA8] (AB130456)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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