Anti-Ctip2 [25B6] FITC conjugated antibody (ab123449) rat monoclonal antibody that is used to detect Ctip2 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.
Images
![Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (AB123449), expandable thumbnail](https://content.abcam.com/products/images/fitc-ctip2-antibody-25b6-ab123449--flow-cytometry-intracellular-img56106.gif "Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (AB123449)")
![Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (AB123449), expandable thumbnail](https://content.abcam.com/products/images/fitc-ctip2-antibody-25b6-ab123449--immunocytochemistry-immunofluorescence-img179715.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (AB123449)")
Publications
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- Cell 180:323-339.e192020Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Del Toro et. al.PubMed 31928845
- Immunity 52:83-95.e42019Differential Expression of the Transcription Factor GATA3 Specifies Lineage and Functions of Innate Lymphoid Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Zhong,Mingzhu Zheng,Kairong Cui,Andrew J Martins,Gangqing Hu,Dan Li,Lino Tessarollo,Serguei Kozlov,Jonathan R Keller,John S Tsang,Keji Zhao,Jinfang ZhuPubMed 31882362
- Scientific reports 9:131542019HIV-1 Vpr mediates the depletion of the cellular repressor CTIP2 to counteract viral gene silencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesF Forouzanfar et. al.PubMed 31511615
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.125-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Zebrafish | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.125-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (PubMed:27959755). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element (By similarity). May also function in the P53-signaling pathway (By similarity).
Alternative names
CTIP2, RIT1, BCL11B, B-cell lymphoma/leukemia 11B, BCL-11B, B-cell CLL/lymphoma 11B, COUP-TF-interacting protein 2, Radiation-induced tumor suppressor gene 1 protein, hRit1
Recommended products
Anti-Ctip2 [25B6] FITC conjugated antibody (ab123449) rat monoclonal antibody that is used to detect Ctip2 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human samples.
Key facts
- Isotype
- IgG2a
- Host species
- Rat
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 25B6
- Purification technique
- Precipitation Ammonium Sulphate
- Concentration
- Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab123449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Fluorochrome/Protein Ratio: at least 3 moles FITC per mole of IgG.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)
ab123449 at 0.125μg/ml staining Ctip2 in Jurkat cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block.The Jurkat cells show a clear CTIP2 specific signal
![Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449), expandable thumbnail](https://content.abcam.com/products/images/fitc-ctip2-antibody-25b6-ab123449--immunocytochemistry-immunofluorescence-img179715.jpg "Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)")
Immunocytochemistry/ Immunofluorescence - FITC Anti-Ctip2 antibody [25B6] (ab123449)
ab123449 staining CTIP2 in Jurkat cells. The cells were fixed with 80% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab123449 at 1/100 dilution (shown in green) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449), expandable thumbnail](https://content.abcam.com/products/images/fitc-ctip2-antibody-25b6-ab123449--flow-cytometry-intracellular-img56107.gif "Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)")
Flow Cytometry (Intracellular) - FITC Anti-Ctip2 antibody [25B6] (ab123449)
ab123449 at 0.125µg/ml staining Ctip2 in SH-SY5Y cells by Flow Cytometry (red) or an equal amount of an FITC conjugated control antibody (black) in 3% BSA block. The SH-SY5Y cells do not show significant CTIP2 signal over the negative control signal.
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