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AB322305

FITC Anti-CXCR5 antibody [EPR23463-30]

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Knockout Tested Rabbit Recombinant Monoclonal CXCR5 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD185, BLR1, MDR15, CXCR5, C-X-C chemokine receptor type 5, CXC-R5, CXCR-5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15, MDR-15

1 Images
Flow Cytometry - FITC Anti-CXCR5 antibody [EPR23463-30] (AB322305)
  • Flow Cyt

Lab

Flow Cytometry - FITC Anti-CXCR5 antibody [EPR23463-30] (AB322305)

Flow cytometry overlay histogram showing wild-type Raji (green line) and CXCR5 knockout Raji stained with ab322305 (magenta line). The cells were incubated in 1x PBS containing 10ug/ml human IgG and 10%; normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab322305) (1x 106 in 100ul at 1 ug/ml (1/500)) for 30min on ice.

Isotype control antibody Rabbit IgG [EPR25A] FITC was used at the same concentration and conditions as the primary antibody (wild-type Raji - black line, CXCR5 knockout Raji - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • 578 PE

    PE Anti-CXCR5 antibody [EPR23463-30]

  • 660 APC

    APC Anti-CXCR5 antibody [EPR23463-30]

  • Unconjugated

    Anti-CXCR5 antibody [EPR23463-30]

  • Carrier free

    Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free

  • Carrier free

    Anti-CXCR5 antibody [EPR23463-30] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23463-30

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We observe only weak staining in human WB. We do not suggest this product for use in IHC with mouse or rat.

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Complementary Products

Assay Kits

AB320049

Human CXCR5 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CXCR5 ELISA Kit (AB320049)

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  • 0
Proteins & Peptides

AB157964

Recombinant Human CXCR5 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CXCR5 protein (GST tag N-Terminus) (AB157964)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CXCR5 also known as CD185 is a chemokine receptor with a mass of approximately 43 kDa. It is part of the G protein-coupled receptor family and is widely expressed on follicular B helper T (Tfh) cells B cells and a subset of circulating blood cells. CXCR5 is recognized as a marker for Tfh cells which are critical for the function and maturation of B cells within germinal centers. In tissues CXCR5 expression appears prominently in regions where immune cell interactions facilitate immune and inflammatory responses.
Biological function summary

CXCR5 serves a significant role in directing B cells and Tfh cells to lymphoid tissues by interacting with its ligand CXCL13. This interaction involves recruitment and organization of B cells into follicles within secondary lymphoid organs. The presence of CXCR5 allows B cells and Tfh cells to navigate the follicular zones efficiently enhance antigen-specific immune responses and contribute to long-lived humoral immunity. CXCR5 functions as part of a complex that includes signaling pathways influencing cellular movements and location.

Pathways

CXCR5 plays a significant part in the immune system's trafficking pathways particularly within the crosstalk between chemokines and their receptors. CXCR5 expression facilitates the CXC chemokine pathway which important for positioning immune cells within lymphoid organs. Additionally CXCR5 interacts with proteins such as CXCL13 working together to pattern the architecture of germinal centers where affinity maturation and class switching of antibodies occur critical processes in adaptive immunity.

CXCR5 has been implicated in autoimmune diseases and certain lymphomas. For example aberrant CXCR5 expression is linked to the pathogenesis of diseases like systemic lupus erythematosus (SLE) where it might contribute to immune dysregulation and chronic inflammation. In the context of certain lymphomas CXCR5's role in lymphoid organ architecture and cell migration becomes even more relevant. Its expression often parallels the presence of CXCL13 hinting at a network of interactions promoting tumor survival and growth within lymphoid landscapes. Understanding these relationships can offer insight into developing therapeutic strategies potentially involving CXCR5 inhibitors or anti-CXCR5 antibodies to address these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
See full target information CXCR5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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