AB52459

FITC Anti-Cytokeratin 18 antibody [C-04]

Name: Anti-Cytokeratin 18 FITC antibody [C-04] (ab52459) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB52459
Price: 510 USD
Availability: InStock

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(16 Publications)

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to FITC. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Cell preparation containing KRT18 protein.

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

5 Images

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Overlay histogram showing intracellular staining of HeLa cells labeled with ab52459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52459, 1μg/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (FITC) (2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

ICC/IF image of ab52459 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52459, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow cytometry (Intracellular) analysis HeLa (human cervix carcinoma cell line) cells labeling Cytokeratin 18 with ab52459 (red). Overlay with Isotype mouse IgG1 control FITC antibody (blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow cytometric intracellular analysis of human peripheral whole blood spiked with HeLa cells labelling Cytokeratin 18 with ab52459 at 1.67 μg/ml.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow cytometric intracellular analysis of HeLa cells (red-filled) from neutrophil granulocytes (black-dashed) labelling Cytokeratin 18 with ab52459 at 1.67 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C-04

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5 µg/mL", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-2 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mammals": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Prior to conjugation, the antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. Purity >95% by SDS-PAGE.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology research 13:729-748 PubMed40249209

2025

Antitumor CD4+ T Helper 1 Cells Target and Control the Outgrowth of Disseminated Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesan Ramamoorthi,Marie Catherine Lee,Carly M Farrell,Colin Snyder,Saurabh K Garg,Amy L Aldrich,Vincent Lok,William Dominguez-Viqueira,Sy K Olson-Mcpeek,Marilin Rosa,Namrata Gautam,Shari Pilon-Thomas,Ling Cen,Krithika N Kodumudi,Doris Wiener,Thordur Oskarsson,Ana P Gomes,Robert A Gatenby,Brian J Czerniecki

International journal of molecular sciences 25: PubMed39519007

2024

Development and Characterization of a Human Mammary Epithelial Cell Culture Model for the Blood-Milk Barrier-A Contribution from the ConcePTION Project.

Applications

Unspecified application

Species

Unspecified reactive species

Debora La Mantia,Nina Nauwelaerts,Chiara Bernardini,Augusta Zannoni,Roberta Salaroli,Qi Lin,Isabelle Huys,Pieter Annaert,Monica Forni

Pharmaceutics 14: PubMed36559089

2022

Encorafenib Acts as a Dual-Activity Chemosensitizer through Its Inhibitory Effect on ABCC1 Transporter In Vitro and Ex Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Dimitrios Vagiannis,Youssif Budagaga,Ziba Sabet,Ivo Hanke,Tomáš Rozkoš,Jakub Hofman

International journal of molecular sciences 23: PubMed36430819

2022

Talazoparib Does Not Interact with ABCB1 Transporter or Cytochrome P450s, but Modulates Multidrug Resistance Mediated by ABCC1 and ABCG2: An in Vitro and Ex Vivo Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ziba Sabet,Dimitrios Vagiannis,Youssif Budagaga,Yu Zhang,Eva Novotná,Ivo Hanke,Tomáš Rozkoš,Jakub Hofman

Translational cancer research 11:2636-2646 PubMed36093536

2022

Detection of circulating tumor cells and evaluation of epithelial-mesenchymal transition patterns of circulating tumor cells in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Xiang Jie,Meng Zhang,Ming Du,Qing-Qing Cai,Qing Cong,Cong-Jian Xu,Xiao-Yan Zhang

Cancer gene therapy 29:1463-1476 PubMed35393570

2022

TMPRSS2-ERG promotes the initiation of prostate cancer by suppressing oncogene-induced senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fang,Dongmei Li,JuanJuan Yin,Hong Pan,Huihui Ye,Joel Bowman,Brian Capaldo,Kathleen Kelly

International journal of molecular sciences 22: PubMed34769363

2021

Tepotinib Inhibits Several Drug Efflux Transporters and Biotransformation Enzymes: The Role in Drug-Drug Interactions and Targeting Cytostatic Resistance In Vitro and Ex Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Vagiannis,Youssif Budagaga,Anselm Morell,Yu Zhang,Eva Novotná,Adam Skarka,Sarah Kammerer,Jan-Heiner Küpper,Ivo Hanke,Tomáš Rozkoš,Jakub Hofman

Animals : an open access journal from MDPI 11: PubMed34359140

2021

Development of a Pig Mammary Epithelial Cell Culture Model as a Non-Clinical Tool for Studying Epithelial Barrier-A Contribution from the IMI-ConcePTION Project.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Bernardini,Debora La Mantia,Roberta Salaroli,Augusta Zannoni,Nina Nauwelaerts,Neel Deferm,Domenico Ventrella,Maria Laura Bacci,Giuseppe Sarli,Michele Bouisset-Leonard,Pieter Annaert,Monica Forni

Animals : an open access journal from MDPI 9: PubMed31640289

2019

Induction of Chemerin on Autophagy and Apoptosis in Dairy Cow Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bianhong Hu,Wenjuan Song,Yujie Tang,Mingyan Shi,Huixia Li,Debing Yu

International journal of molecular sciences 20: PubMed31618976

2019

The Potential of the FSP1cre- Mouse Model for Studying Juvenile NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Jiapeng Chen,Yan Zhuang,Ming Keat Sng,Nguan Soon Tan,Walter Wahli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Cytokeratin 18 antibody [C-04]

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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