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Mouse Monoclonal Cytokeratin 18 antibody - conjugated to FITC. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 18 protein.

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Images

Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459), expandable thumbnail
  • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459), expandable thumbnail
  • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (AB52459), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing Keratin, type I cytoskeletal 18 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow Cyt (Intra)
Human
Tested
Tested
Mammals
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Predicted
Predicted

Species

Mammals

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1.00000-2.00000 µg/mL

Notes

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mammals

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

6 products for Alternative Product

Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

Recommended products

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to FITC. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Cell preparation containing Keratin, type I cytoskeletal 18 protein.

Key facts

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing Keratin, type I cytoskeletal 18 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05783
Clone number

C-04

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Prior to conjugation, the antibody was purified by Protein A affinity chromatography. The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. Purity >95% by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

Product promise

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3 product images

  • Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459), expandable thumbnail

    Immunocytochemistry - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

    ICC/IF image of ab52459 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52459, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459), expandable thumbnail

    Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

    Overlay histogram showing intracellular staining of HeLa cells labeled with ab52459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52459, 1μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (FITC) (2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459), expandable thumbnail

    Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [C-04] (ab52459)

    Flow cytometry (Intracellular) analysis HeLa (human cervix carcinoma cell line) cells labeling Cytokeratin 18 with ab52459 (red). Overlay with Isotype mouse IgG1 control FITC antibody (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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