AB72813

FITC Anti-Cytokeratin 18 antibody [DC-10]

Name: Anti-Cytokeratin 18 FITC antibody [DC-10] (ab72813) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB72813
Price: 520 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to FITC. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing KRT18 protein.

View Alternative Names

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

3 Images

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Overlay histogram showing MCF-7 cells stained with ab72813 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72813, 0.5μg/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (FITC) (2μg/1x10⁶ cells) for 30 min at 22°C. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

ICC/IF image of ab72813 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72813, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Flow cytometry analysis (intracellular staining) of HeLa cells with ab72813 at 5 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DC-10

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.5-1 µg/mL", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "" }, "Cow": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Hamster": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

See full target information KRT18

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:3799-3807 PubMed30881500

2019

Isolation of circulating tumor cells and detection of EGFR mutations in patients with non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Jingying Nong,Jinghui Wang,Zhuohong Yan,Ling Yi,Xin Gao,Zhidong Liu,Hongtao Zhang,Shucai Zhang

International journal of oncology : PubMed28350107

2017

A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Haoqiang Zhang,Peng Gao,Xin Xiao,Michal Heger,Lei Geng,Bo Fan,Yulin Yuan,Chen Huang,Guojing Chen,Yao Liu,Yongchen Hu,Xiuchun Yu,Sujia Wu,Ling Wang,Zhen Wang

Journal of cell science 124:1644-54 PubMed21525033

2011

Phagocytosis of dying tumor cells by human peritoneal mesothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Britta Janina Wagner,Dennis Lindau,Dagmar Ripper,York-Dieter Stierhof,Jörg Glatzle,Maria Witte,Henning Beck,Hildegard Keppeler,Kirsten Lauber,Hans-Georg Rammensee,Alfred Königsrainer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Cytokeratin 18 antibody [DC-10]

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Immunocytochemistry/ Immunofluorescence - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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