AB178543

FITC Anti-Cytokeratin 19 antibody [A53-B/A2]

Name: Anti-Cytokeratin 19 FITC antibody [A53-B/A2] (ab178543) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB178543
Price: 520 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Cytokeratin 19 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing KRT19 protein.

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

2 Images

Flow Cytometry - FITC Anti-Cytokeratin 19 antibody [A53-B/A2] (AB178543)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Cytokeratin 19 antibody [A53-B/A2] (AB178543)

Separation of MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 3 ug/ml, red-filled) from MCF-7 cells stained using mouse IgG2b isotype control (MPC-11) FITC antibody (concentration in sample 3 ug/ml, same as anti-Cytokeratin 19 FITC antibody concentration, black-dashed) in flow cytometry analysis (surface staining).

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 19 antibody [A53-B/A2] (AB178543)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 19 antibody [A53-B/A2] (AB178543)

Flow Cytometry (Intracellular) analysis of MCF-7 human breast adenocarcinoma cell line labeling Cytokeratin 19 with ab178543 at 9 μg/mL (red).

Negative control : Human peripheral whole blood (blue).

Excitation laser : 488 nm

Unconjugated

Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A53-B/A2

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Cell preparation containing KRT19 protein. The exact immunogen used to generate this antibody is proprietary information.

P08727

Specificity

ab178543 reacts with Rod domain of Cytokeratin 19 (40 kDa) in Human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-5 µg/mL", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab178543 is made from a highly purified antibody (>95% by SDS-PAGE) and conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Do Not Freeze|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT19

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:3446-3461 PubMed33637560

2021

A Basomedial Amygdala to Intercalated Cells Microcircuit Expressing PACAP and Its Receptor PAC1 Regulates Contextual Fear.

Applications

Unspecified application

Species

Unspecified reactive species

Abha K Rajbhandari,Christopher J Octeau,Sarah Gonzalez,Zachary T Pennington,Farzanna Mohamed,Jeremy Trott,Jasmine Chavez,Erin Ngyuen,Natasha Keces,Weizhe Z Hong,Rachael L Neve,James Waschek,Baljit S Khakh,Michael S Fanselow

Journal of biological engineering 13:79 PubMed31673286

2019

A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Leila Mohammadzadeh,Reza Rahbarghazi,Roya Salehi,Mehrdad Mahkam

Cell 178:160-175.e27 PubMed31155233

2019

Stromal Microenvironment Shapes the Intratumoral Architecture of Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Ligorio,Srinjoy Sil,Jose Malagon-Lopez,Linda T Nieman,Sandra Misale,Mauro Di Pilato,Richard Y Ebright,Murat N Karabacak,Anupriya S Kulkarni,Ann Liu,Nicole Vincent Jordan,Joseph W Franses,Julia Philipp,Johannes Kreuzer,Niyati Desai,Kshitij S Arora,Mihir Rajurkar,Elad Horwitz,Azfar Neyaz,Eric Tai,Neelima K C Magnus,Kevin D Vo,Chittampalli N Yashaswini,Francesco Marangoni,Myriam Boukhali,Jackson P Fatherree,Leah J Damon,Kristina Xega,Rushil Desai,Melissa Choz,Francesca Bersani,Adam Langenbucher,Vishal Thapar,Robert Morris,Ulrich F Wellner,Oliver Schilling,Michael S Lawrence,Andrew S Liss,Miguel N Rivera,Vikram Deshpande,Cyril H Benes,Shyamala Maheswaran,Daniel A Haber,Carlos Fernandez-Del-Castillo,Cristina R Ferrone,Wilhelm Haas,Martin J Aryee,David T Ting

The American journal of pathology 188:1106-1112 PubMed29454754

2018

Mutational Analysis of AKT1 and PIK3CA in Intraductal Papillomas of the Breast with Special Reference to Cellular Components.

Applications

Unspecified application

Species

Unspecified reactive species

Chieko Mishima,Naofumi Kagara,Jun-Ichiro Ikeda,Eiichi Morii,Tomohiro Miyake,Tomonori Tanei,Yasuto Naoi,Masafumi Shimoda,Kenzo Shimazu,Seung Jin Kim,Shinzaburo Noguchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Cytokeratin 19 antibody [A53-B/A2]

Flow Cytometry - FITC Anti-Cytokeratin 19 antibody [A53-B/A2] (AB178543)

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 19 antibody [A53-B/A2] (AB178543)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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