AB87014

FITC Anti-Cytokeratin 19 antibody [SB39g]

Name: Anti-Cytokeratin 19 FITC antibody [SB39g] (ab87014) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB87014
Price: 520 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal Cytokeratin 19 antibody - conjugated to FITC. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KRT19.

View Alternative Names

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

1 Images

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 19 antibody [SB39g] (AB87014)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 19 antibody [SB39g] (AB87014)

Overlay histogram showing MCF-7 cells stained with ab87014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87014, 0.5μg/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a (FITC) (1μg/1x10⁶ cells) for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SB39g

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC

applications

Immunogen

Recombinant Fragment Protein within Human KRT19. The exact immunogen used to generate this antibody is proprietary information.

P08727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.

This product was changed from ascites to tissue culture supernatant on 17th September 2019. Lot numbers higher than GR3224453 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokeratine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.

Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT19

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed33922695

2021

A Novel Multidrug-Resistant Cell Line from an Italian Intrahepatic Cholangiocarcinoma Patient.

Applications

Unspecified application

Species

Unspecified reactive species

Caterina Peraldo-Neia,Annamaria Massa,Francesca Vita,Marco Basiricò,Chiara Raggi,Paola Bernabei,Paola Ostano,Laura Casorzo,Mara Panero,Francesco Leone,Giuliana Cavalloni,Massimo Aglietta

Current eye research 46:159-167 PubMed32631162

2020

Human Amniotic Epithelial Cells Promote the Proliferation of Human Corneal Endothelial Cells by Regulating Telomerase Activity via the Wnt/β-catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayan Liu,Ye Wen,Wei Luo,Yingying Liu,Xiangyin Sha

Oncoimmunology 7:e1438111 PubMed29900038

2018

Dynamic change of PD-L1 expression on circulating tumor cells in advanced solid tumor patients undergoing PD-1 blockade therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Yue,Yubo Jiang,Ping Li,Yuehua Wang,Jian Xue,Nannan Li,Da Li,Ruina Wang,Yongjun Dang,Zhiyuan Hu,Yanlian Yang,Jianming Xu

Theranostics 7:4710-4721 PubMed29187898

2017

Precisely Enumerating Circulating Tumor Cells Utilizing a Multi-Functional Microfluidic Chip and Unique Image Interpretation Algorithm.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxing Zhou,Hui Zheng,Zhaoba Wang,Ren Li,Xiaoran Liu,Weikai Zhang,Zihua Wang,Huiping Li,Zewen Wei,Zhiyuan Hu

Infectious agents and cancer 10:46 PubMed26672675

2015

Relationship between CK19 expression, deregulation of normal keratinocyte differentiation pattern and high risk-human papilloma virus infection in oral and oropharyngeal squamous cell carcinoma.

Applications

Flow Cyt

Species

Unspecified reactive species

Angela Santoro,Giuseppe Pannone,Rossella Ninivaggi,Massimo Petruzzi,Andrea Santarelli,Giuseppe Maria Russo,Silvia Lepore,Michele Pietrafesa,Ilaria Laurenzana,Rosalia Leonardi,Paolo Bucci,Maria Iole Natalicchio,Alberta Lucchese,Silvana Papagerakis,Pantaleo Bufo

The American journal of pathology 185:1724-39 PubMed25892683

2015

Profiles of cancer stem cell subpopulations in cholangiocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenzo Cardinale,Anastasia Renzi,Guido Carpino,Alessia Torrice,Maria C Bragazzi,Felice Giuliante,Agostino M DeRose,Alice Fraveto,Paolo Onori,Chiara Napoletano,Antonio Franchitto,Alfredo Cantafora,GianLuca Grazi,Nicola Caporaso,Giuseppe D'Argenio,Gianfranco Alpini,Lola M Reid,Eugenio Gaudio,Domenico Alvaro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Cytokeratin 19 antibody [SB39g]

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 19 antibody [SB39g] (AB87014)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Product promise