AB2492

FITC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Name: Anti-DDDDK tag (Binds to FLAG® tag sequence) FITC antibody (ab2492) | Abcam
Brand: Abcam Limited
SKU: AB2492
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to FITC. Suitable for WB and reacts with Tag samples. Cited in 5 publications.

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Applications

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

The molar F/P ratio is 4.1.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature chemical biology 17:1057-1064 PubMed34168368

2021

Rapid generation of potent antibodies by autonomous hypermutation in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Alon Wellner,Conor McMahon,Morgan S A Gilman,Jonathan R Clements,Sarah Clark,Kianna M Nguyen,Ming H Ho,Vincent J Hu,Jung-Eun Shin,Jared Feldman,Blake M Hauser,Timothy M Caradonna,Laura M Wingler,Aaron G Schmidt,Debora S Marks,Jonathan Abraham,Andrew C Kruse,Chang C Liu

Cell 184:2454-2470.e26 PubMed33857425

2021

Glioblastomas acquire myeloid-affiliated transcriptional programs via epigenetic immunoediting to elicit immune evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Gangoso,Benjamin Southgate,Leanne Bradley,Stefanie Rus,Felipe Galvez-Cancino,Niamh McGivern,Esra Güç,Chantriolnt-Andreas Kapourani,Adam Byron,Kirsty M Ferguson,Neza Alfazema,Gillian Morrison,Vivien Grant,Carla Blin,IengFong Sou,Maria Angeles Marques-Torrejon,Lucia Conde,Simona Parrinello,Javier Herrero,Stephan Beck,Sebastian Brandner,Paul M Brennan,Paul Bertone,Jeffrey W Pollard,Sergio A Quezada,Duncan Sproul,Margaret C Frame,Alan Serrels,Steven M Pollard

JCI insight 4: PubMed31391342

2019

An Hb-mediated circulating macrophage contributing to pulmonary vascular remodeling in sickle cell disease.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Redinus,Jin Hyen Baek,Ayla Yalamanoglu,Hye Kyung H Shin,Radu Moldova,Julie W Harral,Delaney Swindle,David Pak,Scott K Ferguson,Rachelle Nuss,Kathryn Hassell,Eva Nozik-Grayck,Andre F Palmer,Mehdi A Fini,Vijaya Karoor,Kurt R Stenmark,Paul W Buehler,David C Irwin

Nature communications 9:1842 PubMed29743476

2018

Sema7A/PlxnCl signaling triggers activity-dependent olfactory synapse formation.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuko Inoue,Hirofumi Nishizumi,Hiromi Naritsuka,Hiroshi Kiyonari,Hitoshi Sakano

Oncology letters 13:949-954 PubMed28356983

2017

TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma and .

Applications

Unspecified application

Species

Unspecified reactive species

Qing Guo,Jia He,Feng Shen,Wei Zhang,Xi Yang,Chi Zhang,Qu Zhang,Jun-Xing Huang,Zheng-Dong Wu,Xin-Chen Sun,Sheng-Bin Dai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (5)

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