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AB183393

FITC Anti-DNA/RNA Damage antibody [15A3]

5

(1 Review)

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(11 Publications)

Anti-DNA/RNA Damage antibody [15A3] - FITC conjugated (ab183393) is a mouse monoclonal antibody detecting DNA/RNA Damage in IHC-P, IHC-Fr, ICC/IF.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393)

Immunohistochemical analysis of formalin-fixed mouse inflamed colon labelling DNA damage with ab183393 at 1/1000000 dilution, followed by Biotin Goat Anti-Mouse at 1/2000 dilution. Counterstained with Mayer Hematoxylin (purple/blue) nuclear stain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393)

Immunohistochemical analysis of paraffin-embedded mouse back skin tissue, labelling DNA damage with ab62623 (non-conjugated antibody clone) at 1/100 dilution, followed by FITC Goat Anti-Mouse (green) at 1/50 dilution at RT for 1 hour.

  • Unconjugated

    Anti-DNA/RNA Damage antibody [15A3]

  • Biotin

    Biotin Anti-DNA/RNA Damage antibody [15A3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15A3

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

IHC-P, IHC-Fr, ICC/IF

applications

Specificity

15A3 binds with high specificity and affinity to oxo8dG (8-hydroxy-2'-deoxyguanosine), oxo8Gua (8-oxo-7,8-dihydroguanine), and oxo8G (8-oxo-7,8-dihydroguanosine) present in biological fluids with Kaff values of 9.2 x 108 M-1, 4.7 x' 17 M-1, and 2.1 x 108 M-1, respectively.

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Alternative Products

Primary Antibodies

AB183395

Biotin Anti-DNA/RNA Damage antibody [15A3]

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB62623

Anti-DNA/RNA Damage antibody [15A3]

Immunocytochemistry - Anti-DNA/RNA Damage antibody [15A3] (AB62623)

  • 4
  • 14
Primary Antibodies

AB46545

Anti-4 Hydroxynonenal antibody

Western blot - Anti-4 Hydroxynonenal antibody (AB46545)

  • 4
  • 27
Primary Antibodies

AB48508

Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (AB48508)

  • 5
  • 10
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nucleic Acid": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1-10 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1-10 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF). Related products
Antibody clone 15A3 is also available pre-conjugated to a variety of labels for your convenience – Anti-DNA/RNA Damage FITC [15A3] (ab183393).

Other related products
We have a range of other formats of antibody clone [15A3] also available for your convenience: ab62623, FITC - ab183393, Biotin - ab183395
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DNA/RNA Damage
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2897:331-340 PubMed40202646

2025

Assessment of the Sperm DNA Oxidative Damage by Immunofluorescence and Flow Cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Estíbaliz Lacalle,Estela Fernández-Alegre,Belén Gómez-Giménez,Felipe Martínez-Pastor,Cristina Soriano-Úbeda

Frontiers in physiology 15:1474933 PubMed39493864

2024

Hyperbaric oxygen-induced acute lung injury: A mouse model study on pathogenic characteristics and recovery dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Wang,Hong Chen,Zhi Li,Guangxu Xu,Xiaochen Bao

Nature communications 14:7856 PubMed38030636

2023

A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjian Huang,Rong Yang,Jiao Jiao,Ze Li,Penghui Wang,Ye Liu,Sicheng Li,Canwen Chen,Zongan Li,Guiwen Qu,Kang Chen,Xiuwen Wu,Bo Chi,Jianan Ren

Antioxidants (Basel, Switzerland) 12: PubMed37237912

2023

Loss of Nuclear/DNA Integrity in Mouse Epididymal Spermatozoa after Short-Term Exposure to Low Doses of Dibutyl Phthalate or Bisphenol AF and Its Mitigation by Oral Antioxidant Supplementation.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Hug,Pauline Villeneuve,Stephanie Bravard,Areski Chorfa,Christelle Damon-Soubeyrand,Stephen G Somkuti,Aron Moazamian,R John Aitken,Parviz Gharagozloo,Joël R Drevet,Fabrice Saez

Cancer cell 41:356-372.e10 PubMed36706760

2023

Neutrophil-activating therapy for the treatment of cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ian L Linde,Tyler R Prestwood,Jingtao Qiu,Genay Pilarowski,Miles H Linde,Xiangyue Zhang,Lei Shen,Nathan E Reticker-Flynn,David Kung-Chun Chiu,Lauren Y Sheu,Simon Van Deursen,Lorna L Tolentino,Wen-Chao Song,Edgar G Engleman

Orthopaedic surgery 13:2145-2152 PubMed34559465

2021

Oxidative Stress Induced Osteocyte Apoptosis in Steroid-Induced Femoral Head Necrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Qi Fan,Shu-Cai Bai,Qian Xu,Zhi-Jun Li,Wen-Hao Cui,Hui Li,Xiao-Hui Li,Hua-Feng Zhang

Frontiers in physiology 12:662833 PubMed34335290

2021

Antioxidant and Anti-atherogenic Properties of and Aqueous Extracts: Modulation of NADPH Oxidase-Derived Reactive Oxygen Species.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Quesada,Matilde de Paola,María Soledad Alvarez,María Belén Hapon,Carlos Gamarra-Luques,Claudia Castro

PloS one 15:e0232724 PubMed32374749

2020

Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hirofumi Kurita,Natsuki Haruta,Yoshito Uchihashi,Takahito Seto,Kazunori Takashima

Scientific reports 10:2896 PubMed32076073

2020

Fisetin protects against cardiac cell death through reduction of ROS production and caspases activity.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Rodius,Niek de Klein,Céline Jeanty,Héctor Sánchez-Iranzo,Isaac Crespo,Mark Ibberson,Ioannis Xenarios,Gunnar Dittmar,Nadia Mercader,Simone P Niclou,Francisco Azuaje

Redox biology 15:316-326 PubMed29306194

2018

Downregulation of PARP1 transcription by CDK4/6 inhibitors sensitizes human lung cancer cells to anticancer drug-induced death by impairing OGG1-dependent base excision repair.

Applications

Unspecified application

Species

Unspecified reactive species

Dominika Tempka,Paulina Tokarz,Kinga Chmielewska,Magdalena Kluska,Julita Pietrzak,Żaneta Rygielska,László Virág,Agnieszka Robaszkiewicz
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com