Anti-DNA/RNA Damage [15A3] FITC conjugated antibody (ab183393) mouse monoclonal antibody that is used to detect DNA/RNA Damage in IHC-P, IHC-Fr, ICC/IF.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393), expandable thumbnail](https://content.abcam.com/products/images/fitc-dna-rna-damage-antibody-15a3-ab183393--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165044.png/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393), expandable thumbnail](https://content.abcam.com/products/images/fitc-dna-rna-damage-antibody-15a3-ab183393--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174828.png/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (AB183393)")
Publications
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- Nature communications 14:78562023A click chemistry-mediated all-peptide cell printing hydrogel platform for diabetic wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinjian Huang,Rong Yang,Jiao Jiao,Ze Li,Penghui Wang,Ye Liu,Sicheng Li,Canwen Chen,Zongan Li,Guiwen Qu,Kang Chen,Xiuwen Wu,Bo Chi,Jianan RenPubMed 38030636
- Antioxidants (Basel, Switzerland) 12:2023Loss of Nuclear/DNA Integrity in Mouse Epididymal Spermatozoa after Short-Term Exposure to Low Doses of Dibutyl Phthalate or Bisphenol AF and Its Mitigation by Oral Antioxidant Supplementation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Hug,Pauline Villeneuve,Stephanie Bravard,Areski Chorfa,Christelle Damon-Soubeyrand,Stephen G Somkuti,Aron Moazamian,R John Aitken,Parviz Gharagozloo,Joël R Drevet,Fabrice SaezPubMed 37237912
- Orthopaedic surgery 13:2145-21522021Oxidative Stress Induced Osteocyte Apoptosis in Steroid-Induced Femoral Head Necrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Qi Fan,Shu-Cai Bai,Qian Xu,Zhi-Jun Li,Wen-Hao Cui,Hui Li,Xiao-Hui Li,Hua-Feng ZhangPubMed 34559465
- Frontiers in physiology 12:6628332021Antioxidant and Anti-atherogenic Properties of and Aqueous Extracts: Modulation of NADPH Oxidase-Derived Reactive Oxygen Species.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsabel Quesada,Matilde de Paola,María Soledad Alvarez,María Belén Hapon,Carlos Gamarra-Luques,Claudia CastroPubMed 34335290
- PloS one 15:e02327242020Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHirofumi Kurita,Natsuki Haruta,Yoshito Uchihashi,Takahito Seto,Kazunori TakashimaPubMed 32374749
- Scientific reports 10:28962020Fisetin protects against cardiac cell death through reduction of ROS production and caspases activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Rodius et. al.PubMed 32076073
- Redox biology 15:316-3262018Downregulation of PARP1 transcription by CDK4/6 inhibitors sensitizes human lung cancer cells to anticancer drug-induced death by impairing OGG1-dependent base excision repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominika Tempka,Paulina Tokarz,Kinga Chmielewska,Magdalena Kluska,Julita Pietrzak,Żaneta Rygielska,László Virág,Agnieszka RobaszkiewiczPubMed 29306194
- Proceedings of the National Academy of Sciences of the United States of America 89:3375-91992Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE M Park,M K Shigenaga,P Degan,T S Korn,J W Kitzler,C M Wehr,P Kolachana,B N AmesPubMed 1565629
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Chemical / Small Molecule corresponding to DNA/RNA Damage.
Reactivity data
Select an application| IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|
| Nucleic Acid | Expected | Expected | Expected |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Nucleic Acid | Dilution info 1.00000-10.00000 µg/mL | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Nucleic Acid | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Nucleic Acid | Dilution info 1.00000-10.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Alternative names
7,8-Dihydro-8-oxo-2'-deoxyguanosine, 7,8-Dihydro-8-oxodeoxyguanosine, 7,8-Dihydro-8-oxoguanine, 8-Hydroxy-2'-deoxyguanosine, 8-Hydroxydeoxyguanosine, 8-OH-dG, 8-Oxo-2'-deoxyguanosine, 8-Oxo-7,8-dihydro-2'-deoxyguanosine (Oxo-8-dG), 8-Oxo-7,8-dihydrodeoxyguanosine, 8-Oxo-G, 8-Oxo-dG, 8-Oxo-guanosine, 8-Oxo-riboguanosine, 8-Oxoguanine, 8-hydroxyguanine, 8-oxo-7,8-dihydroguanine (Oxo-8-Gua), 8-oxo-7,8-dihydroguanosine (Oxo-8-G), 8-oxo-Gua, OH8Gua
Recommended products
Anti-DNA/RNA Damage [15A3] FITC conjugated antibody (ab183393) mouse monoclonal antibody that is used to detect DNA/RNA Damage in IHC-P, IHC-Fr, ICC/IF.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Chemical / Small Molecule corresponding to DNA/RNA Damage.
- Clone number
- 15A3
- Purification technique
- Affinity purification Protein G
- Specificity
15A3 binds with high specificity and affinity to oxo8dG (8-hydroxy-2'-deoxyguanosine), oxo8Gua (8-oxo-7,8-dihydroguanine), and oxo8G (8-oxo-7,8-dihydroguanosine) present in biological fluids with Kaff values of 9.2 x 108 M-1, 4.7 x' 17 M-1, and 2.1 x 108 M-1, respectively.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), .
Related products
Antibody clone 15A3 is also available pre-conjugated to a variety of labels for your convenience – Anti-DNA/RNA Damage FITC [15A3] (ab183393).
Other related products
We have a range of other formats of antibody clone [15A3] also available for your convenience:
Anti-DNA/RNA Damage antibody [15A3] ab62623, FITC - ab183393, Biotin - Biotin Anti-DNA/RNA Damage antibody [15A3] ab183395
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393), expandable thumbnail](https://content.abcam.com/products/images/fitc-dna-rna-damage-antibody-15a3-ab183393--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165044.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393)
Immunohistochemical analysis of formalin-fixed mouse inflamed colon labelling DNA damage with ab183393 at 1/1000000 dilution, followed by Biotin Goat Anti-Mouse at 1/2000 dilution. Counterstained with Mayer Hematoxylin (purple/blue) nuclear stain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393), expandable thumbnail](https://content.abcam.com/products/images/fitc-dna-rna-damage-antibody-15a3-ab183393--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174828.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-DNA/RNA Damage antibody [15A3] (ab183393)
Immunohistochemical analysis of paraffin-embedded mouse back skin tissue, labelling DNA damage with Anti-DNA/RNA Damage antibody [15A3] ab62623 (non-conjugated antibody clone) at 1/100 dilution, followed by FITC Goat Anti-Mouse (green) at 1/50 dilution at RT for 1 hour.
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