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Rat Monoclonal EGFR antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human EGFR.


Images

Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (AB11400), expandable thumbnail
  • Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (AB11400), expandable thumbnail
  • Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (AB11400), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Native Full Length Protein corresponding to Human EGFR. Database link P00533

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50.00000 - 1/100.00000
Notes

Use 10ul of the suggested working dilution to label 106cells in 100ul.

ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

7 products for Alternative Product

Target data

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029, PubMed:20837704, PubMed:27153536, PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Alternative names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Recommended products

Rat Monoclonal EGFR antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human EGFR.

Key facts

Isotype
IgG2a
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Native Full Length Protein corresponding to Human EGFR. Database link P00533
Clone number
ICR10
Purification technique
Ion exchange chromatography
Epitope
Binds Epitope B with an affinity of 6.7 x 10-9 M.
Concentration
Loading...
Purification notes

Purified IgG prepared by ion exchange chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This product should be stored undiluted.
Storage in frost-free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

Associated diseases and disorders

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

Product promise

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3 product images

  • Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (ab11400), expandable thumbnail

    Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (ab11400)

    Flow Cytometry analysis of A431 cells labeling EGFR with ab11400.

  • Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (ab11400), expandable thumbnail

    Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (ab11400)

    Flow Cytometry analysis of A431 cells labeling EGFR with ab11400.

  • Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (ab11400), expandable thumbnail

    Flow Cytometry - FITC Anti-EGFR antibody [ICR10] (ab11400)

    ab11400 (1/10 dilution) staining A431 cells by Flow Cytometry. Green - isotype control; Red - ab11400.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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