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AB112067

FITC Anti-EpCAM antibody [VU-1D9]

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(1 Review)

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(5 Publications)

Mouse Monoclonal EPCAM antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

View Alternative Names

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

1 Images
Flow Cytometry - FITC Anti-EpCAM antibody [VU-1D9] (AB112067)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-EpCAM antibody [VU-1D9] (AB112067)

Flow cytometry analysis (surface staining) of human MCF-7 (red) and SP2 (black) cell lines with ab112067.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

VU-1D9

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

Specificity

ab112067 strongly stains various normal epithelial cells and carcinomas.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>20 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }
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Product details

The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
See full target information EPCAM
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature biomedical engineering 9:127-148 PubMed39633028

2024

Arrayed CRISPR libraries for the genome-wide activation, deletion and silencing of human protein-coding genes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-An Yin,Lukas Frick,Manuel C Scheidmann,Tingting Liu,Chiara Trevisan,Ashutosh Dhingra,Anna Spinelli,Yancheng Wu,Longping Yao,Dalila Laura Vena,Britta Knapp,Jingjing Guo,Elena De Cecco,Kathi Ging,Andrea Armani,Edward J Oakeley,Florian Nigsch,Joel Jenzer,Jasmin Haegele,Michal Pikusa,Joachim Täger,Salvador Rodriguez-Nieto,Vangelis Bouris,Rafaela Ribeiro,Federico Baroni,Manmeet Sakshi Bedi,Scott Berry,Marco Losa,Simone Hornemann,Martin Kampmann,Lucas Pelkmans,Dominic Hoepfner,Peter Heutink,Adriano Aguzzi

Materials today. Bio 26:101061 PubMed38711937

2024

3D in vitro synovial hyperplasia model on polycaprolactone-micropatterned nanofibrous microwells for screening disease-modifying anti-rheumatic drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Dongwoo Kim,Jiyeon Heo,Boa Song,Gyubok Lee,Changgi Hong,Zhuomin Jiang,Sohui Lee,Kangwon Lee,Mingyo Kim,Min Hee Park

Journal of the American Society of Nephrology : JASN 33:949-965 PubMed35197326

2022

Serum Protein Exposure Activates a Core Regulatory Program Driving Human Proximal Tubule Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin A Lidberg,Selvaraj Muthusamy,Mohamed Adil,Anish Mahadeo,Jade Yang,Ranita S Patel,Lu Wang,Theo K Bammler,Jonathan Reichel,Catherine K Yeung,Jonathan Himmelfarb,Edward J Kelly,Shreeram Akilesh

ACS applied materials & interfaces 9:29571-29579 PubMed28805365

2017

Removal of Cells from Body Fluids by Magnetic Separation in Batch and Continuous Mode: Influence of Bead Size, Concentration, and Contact Time.

Applications

Unspecified application

Species

Unspecified reactive species

Nils Bohmer,Nino Demarmels,Elena Tsolaki,Lukas Gerken,Kerda Keevend,Sergio Bertazzo,Marco Lattuada,Inge K Herrmann

Analytical chemistry 86:6355-62 PubMed24927449

2014

Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin P Casavant,David J Guckenberger,David J Beebe,Scott M Berry
View all publications
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