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AB279601

FITC Anti-Eph receptor B4/HTK antibody [04]

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(1 Publication)

Mouse Monoclonal Eph receptor B4/HTK antibody - conjugated to FITC. Suitable for ICC, Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EPHB4 aa 1 to C-terminus.

View Alternative Names

HTK, MYK1, TYRO11, EPHB4, Ephrin type-B receptor 4, Hepatoma transmembrane kinase, Tyrosine-protein kinase TYRO11

2 Images
Flow Cytometry - FITC Anti-Eph receptor B4/HTK antibody [04] (AB279601)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Eph receptor B4/HTK antibody [04] (AB279601)

Flow cytometric analysis of EphB4 expression on MCF-7 cells. Cells were stained with ab279601 (black peak) along with an isotype control (grey peak).

Immunocytochemistry - FITC Anti-Eph receptor B4/HTK antibody [04] (AB279601)
  • ICC

Supplier Data

Immunocytochemistry - FITC Anti-Eph receptor B4/HTK antibody [04] (AB279601)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 1% Triton X-100 permeabilized and 10% serum blocked HeLa cells stained for EphB4 using ab279601 (green) at 10 μg/ml concentration at 37℃ 1 hour. Positive staining was localized to plasma membrane. Nuclear staining in blue.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

04

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC

applications

Immunogen

Recombinant Fragment Protein within Human EPHB4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P54760

Specificity

Human EphB4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "5-20 g/mL", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Concentration: 10 μl/Test, 0.1 mg/ml</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 99.41% Deionized Water (H20), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Eph receptor B4 also known as EphB4 or HTK is a protein belonging to the Ephrin receptor subfamily of receptor tyrosine kinases. The receptor has a molecular weight of about 120 kDa. EphB4 typically interacts with its binding partner ephrin-B2 facilitating bidirectional signaling. These complexes reside in the cell membrane and are mainly found in vascular endothelial cells and various tissues including the heart lungs and skin. Notably this receptor plays a role in cellular processes like cell migration and adhesion.
Biological function summary

The Eph receptor B4 is a significant regulator of angiogenesis and vascular development. It forms a critical component of a signaling complex that also includes ephrin-B2. During embryonic development EphB4's interaction with ephrin-B2 guides the proper formation and organization of blood vessels. In adult organisms it remains active in maintaining vascular homeostasis. Additionally this receptor impacts neuronal development and repair through modulating axon guidance and synaptic plasticity.

Pathways

EphB4 engages in various cellular signaling cascades. It features prominently within pathways governing angiogenesis and cell adhesion. EphB4 acts as a central player in the VEGF signaling pathway modulating endothelial cell behavior. The protein’s pathways frequently intersect with other proteins such as FAK and Src influencing cell motility and morphology. Furthermore in the ephrin receptor pathway it cross-talks with other Eph receptors like EphA4 assisting in diverse biological functions.

Abnormal Eph receptor B4 activity associates with several pathological conditions. Dysregulation often links to cancer due to its role in neovascularization and tumor progression. Overexpression of EphB4 in tumors like breast and colorectal cancer suggests a contribution to malignancy by supporting angiogenesis and tumor growth. In vascular disorders impaired EphB4-ephrin-B2 signaling may lead to defects in blood vessel formation. Additionally alterations in this receptor are observed in neurological diseases such as Alzheimer's where EphB4 interacts with tau protein-modifying vascular and neuronal deficiencies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 it is involved in the regulation of cell adhesion and migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells.
See full target information EPHB4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 15: PubMed36398590

2022

Bortezomib-induced neurotoxicity in human neurons is the consequence of nicotinamide adenine dinucleotide depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew R Snavely,Keungjung Heo,Veselina Petrova,Tammy Szu-Yu Ho,Xuan Huang,Crystal Hermawan,Ruth Kagan,Tao Deng,Ilyas Singeç,Long Chen,Lee B Barret,Clifford J Woolf
View all publications
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Product promise

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