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AB60343

FITC Anti-F4/80 antibody [BM8]

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(42 Publications)

Rat Monoclonal Adgre1 antibody - conjugated to FITC. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples. Cited in 42 publications. Immunogen corresponding to Cell preparation containing Adgre1 protein.

View Alternative Names

Adgre1

2 Images
Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (AB60343)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-F4/80 antibody [BM8] (AB60343)

Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2μg/1x106 cells) with secondary antibody DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in RAW 264.7 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (AB60343)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FITC Anti-F4/80 antibody [BM8] (AB60343)

ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1μg/ml, FITC conjugated) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

BM8

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.
Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Adgre1
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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 28:79 PubMed37828427

2023

Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huimeng Qi,Zhaoguo Zheng,Qiang Liu

BMC biotechnology 23:43 PubMed37789318

2023

Engineered FGF19 protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Shi,Tiantian Zhao,Lei Huang,Xiaomin Pan,Tianzhen Wu,Xin Feng,Taoli Chen,Jiamin Wu,Jianlou Niu

Nature communications 14:1213 PubMed36869026

2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lai Yee Cheong,Baile Wang,Qin Wang,Leigang Jin,Kelvin H M Kwok,Xiaoping Wu,Lingling Shu,Huige Lin,Sookja Kim Chung,Kenneth K Y Cheng,Ruby L C Hoo,Aimin Xu

iScience 26:106254 PubMed36949755

2023

LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Hernandez,Chia-Lin Chen,Tatsuya Machida,Dinesh Babu Uthaya Kumar,Stanley M Tahara,Jared Montana,Linda Sher,Jake Liang,Jae U Jung,Hidekazu Tsukamoto,Keigo Machida

Journal of cardiovascular pharmacology 80:609-615 PubMed35881902

2022

Sestrin2 Is Increased in Calcific Aortic Disease and Inhibits Osteoblastic Differentiation in Valvular Interstitial Cells via the Nuclear Factor E2-related Factor 2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haixiong Wang,Jicheng Xi,Zhibiao Zhang,Jun Li,Liping Guo,Na Li,Yuehui Sun,Xiaofang Li,Xuebin Han

Nature communications 13:5700 PubMed36171212

2022

In vivo self-assembled siRNA as a modality for combination therapy of ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyan Zhou,Mengchao Yu,Luzhen Ma,Jinyu Fu,Jingwei Guo,Jieqiong Lei,Zheng Fu,Yong Fu,Qipeng Zhang,Chen-Yu Zhang,Xi Chen

Open life sciences 17:1104-1115 PubMed36133421

2022

Interleukin-17A influences the vulnerability rather than the size of established atherosclerotic plaques in apolipoprotein E-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wang,Xitan Hou,Yaning Sun,Chao Lei,Sha Yang,Yao Zhu,Yingming Jiang,Li Song

Frontiers in pharmacology 13:934136 PubMed36059948

2022

Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice.

Applications

Unspecified application

Species

Unspecified reactive species

Panshuang Qiao,Yingli Jia,Ang Ma,Jinzhao He,Chen Shao,Xiaowei Li,Shuyuan Wang,Baoxue Yang,Hong Zhou

Purinergic signalling 18:317-343 PubMed35779163

2022

Lysophosphatidylcholine induces adenosine release from macrophages via TRPM7-mediated mitochondrial activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Youssef,Dong-Keun Song

Frontiers in pharmacology 13:887263 PubMed35721117

2022

Dihydromyricetin-Encapsulated Liposomes Inhibit Exhaustive Exercise-Induced Liver Inflammation by Orchestrating M1/M2 Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Zhou,Long Yi,Hedong Lang,Jun Zhang,Qianyong Zhang,Li Yu,Jundong Zhu,Mantian Mi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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