AB105155

FITC Anti-F4/80 antibody [CI:A3-1]

Name: Anti-F4/80 FITC antibody [CI:A3-1] (ab105155) Rat monoclonal IgG2b | Abcam
Brand: Abcam Limited
SKU: AB105155
Price: 525 USD
Availability: InStock

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(18 Publications)

Rat Monoclonal F4/80 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 18 publications. Immunogen corresponding to Cell preparation containing Adgre1 protein.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

2 Images

Flow Cytometry - FITC Anti-F4/80 antibody [CI:A3-1] (AB105155)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-F4/80 antibody [CI:A3-1] (AB105155)

Flow cytometry staining of mouse peritoneal macrophages with ab105155.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-F4/80 antibody [CI:A3-1] (AB105155)

FSC/SSC gated mouse peritoneal macrophages dual stained with ab105155 at 1/5 dilution and anti-CD11b at 1/5 dilution by Flow cytometry. Isotype control pair in red. Fc receptors were blocked.

Unconjugated

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker
578 PE

PE Anti-F4/80 antibody [CI:A3-1]
660 APC

APC Anti-F4/80 antibody [CI:A3-1]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CI:A3-1

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q61549

Specificity

ab105155 recognises the murine F4/80 antigen.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>in 100µl.</p><p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Journal of hepatocellular carcinoma 10:1019-1035 PubMed37435155

2023

LncRNA MEG3 Inhibits Tumor Progression by Modulating Macrophage Phenotypic Polarization via miR-145-5p/DAB2 Axis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wei,Guoman Liu,Zihua Huang,Yanyan Huang,Lizheng Huang,Zheng Huang,Xianjian Wu,Huamei Wei,Jian Pu

Stem cell research & therapy 13:113 PubMed35313964

2022

Exendin-4 and eldecalcitol synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells through M2 macrophages polarization via PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yupu Lu,Shanshan Liu,Panpan Yang,Yuying Kou,Congshan Li,Hongrui Liu,Minqi Li

Journal of experimental & clinical cancer research : CR 41:1 PubMed34980222

2022

Prevotellaceae produces butyrate to alleviate PD-1/PD-L1 inhibitor-related cardiotoxicity via PPARα-CYP4X1 axis in colonic macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxin Chen,Yanzhuo Liu,Yang Wang,Xuewei Chen,Chenlong Wang,Xuehan Chen,Xi Yuan,Lilong Liu,Jing Yang,Xiaoyang Zhou

Oxidative medicine and cellular longevity 2021:9959746 PubMed34745428

2021

M1 Bone Marrow-Derived Macrophage-Derived Extracellular Vesicles Inhibit Angiogenesis and Myocardial Regeneration Following Myocardial Infarction via the MALAT1/MicroRNA-25-3p/CDC42 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bairong Chen,Liyun Luo,Xiaoliang Wei,Dong Gong,Zhihui Li,Songbiao Li,Wenyi Tang,Lizi Jin

The American journal of pathology 190:372-387 PubMed31843499

2019

Inflammation and Ectopic Fat Deposition in the Aging Murine Liver Is Influenced by CCR2.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth C Stahl,Evan R Delgado,Frances Alencastro,Samuel T LoPresti,Patrick D Wilkinson,Nairita Roy,Martin J Haschak,Clint D Skillen,Satdarshan P Monga,Andrew W Duncan,Bryan N Brown

Advanced science (Weinheim, Baden-Wurttemberg, Ger 6:1900513 PubMed31637157

2019

Exosome-Guided Phenotypic Switch of M1 to M2 Macrophages for Cutaneous Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Hyosuk Kim,Sun Young Wang,Gijung Kwak,Yoosoo Yang,Ick Chan Kwon,Sun Hwa Kim

Nutrition & metabolism 16:49 PubMed31372176

2019

Apoptosis and metastasis inhibitory potential of pineapple vinegar against mouse mammary gland cells in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Nurul Elyani Mohamad,Nadiah Abu,Swee Keong Yeap,Kian Lam Lim,Muhammad Firdaus Romli,Shaiful Adzni Sharifuddin,Kamariah Long,Noorjahan Banu Alitheen

Molecular therapy : the journal of the American So 26:2738-2750 PubMed30301668

2018

4Mu Decreases CD47 Expression on Hepatic Cancer Stem Cells and Primes a Potent Antitumor T Cell Response Induced by Interleukin-12.

Applications

Unspecified application

Species

Unspecified reactive species

Marcelo M Rodríguez,Esteban Fiore,Juan Bayo,Catalina Atorrasagasti,Mariana García,Agostina Onorato,Luciana Domínguez,Mariana Malvicini,Guillermo Mazzolini

Molecular medicine reports 18:4388-4398 PubMed30221697

2018

Streptozotocin‑induced diabetic mice exhibit reduced experimental choroidal neovascularization but not corneal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Gaoqin Liu,Lei Chen,Qinhua Cai,Hongya Wu,Zhigang Chen,Xueguang Zhang,Peirong Lu

Cell metabolism 28:907-921.e7 PubMed30174308

2018

Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.

Applications

Unspecified application

Species

Unspecified reactive species

Salvatore Fabbiano,Nicolas Suárez-Zamorano,Claire Chevalier,Vladimir Lazarević,Silas Kieser,Dorothée Rigo,Stefano Leo,Christelle Veyrat-Durebex,Nadia Gaïa,Marcello Maresca,Doron Merkler,Mercedes Gomez de Agüero,Andrew Macpherson,Jacques Schrenzel,Mirko Trajkovski

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-F4/80 antibody [CI:A3-1]

Flow Cytometry - FITC Anti-F4/80 antibody [CI:A3-1] (AB105155)

Flow Cytometry - FITC Anti-F4/80 antibody [CI:A3-1] (AB105155)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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