Mouse Monoclonal Fas antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Rhesus monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Human FAS.
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![Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (AB33227), expandable thumbnail](https://content.abcam.com/products/images/fitc-fas-antibody-lob-3-17-ab33227--flow-cytometry-img3643.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (AB33227)")
Publications
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- Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 18:1741-72003TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinn-Yang Chen,Chin-Wen Chi,Hui-Ling Chen,Chiung-Pei Wan,Wu-Chang Yang,An-Hang YangPubMed 12937219
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Protein within Human FAS. The exact immunogen used to generate this antibody is proprietary information. Database link P25445
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Rhesus monkey | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1.00000 - 1/10.00000 for 106 Cells | Notes Use 10μl of the suggested working dilution to label 106 cells in 100μl. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes Use 10μl of the suggested working dilution to label 106 cells in 100μl. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
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Target data
Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Alternative names
CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor
Recommended products
Mouse Monoclonal Fas antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Rhesus monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Human FAS.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- FITC
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Protein within Human FAS. The exact immunogen used to generate this antibody is proprietary information. Database link P25445
- Clone number
- LOB 3/17
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (ab33227), expandable thumbnail](https://content.abcam.com/products/images/fitc-fas-antibody-lob-3-17-ab33227--flow-cytometry-img3643.jpg "Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (ab33227)")
Flow Cytometry - FITC Anti-Fas antibody [LOB 3/17] (ab33227)
ab33227, used neat or at a dilution of 1/10 (and then using 10µl of the suggested working dilution to label 106 cells in 100µl), staining CD95 in HUT78 T cell line by Flow Cytometry.
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