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AB33227

FITC Anti-Fas antibody [LOB3/17]

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(1 Publication)

Mouse Monoclonal Fas antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Rhesus monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Protein within Human FAS.

View Alternative Names

CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

1 Images
Flow Cytometry - FITC Anti-Fas antibody [LOB3/17] (AB33227)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Fas antibody [LOB3/17] (AB33227)

ab33227, used neat or at a dilution of 1/10 (and then using 10µl of the suggested working dilution to label 106 cells in 100µl), staining CD95 in HUT78 T cell line by Flow Cytometry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LOB 3/17

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Rhesus monkey

Applications

Flow Cyt

applications

Immunogen

Recombinant Protein within Human FAS. The exact immunogen used to generate this antibody is proprietary information.

P25445

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/1 - 1/10 for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl.</p><p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Rhesus monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Use 10μl of the suggested working dilution to label 10<sup>6</sup> cells in 100μl.</p><p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
See full target information FAS

Publications (1)

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Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 18:1741-7 PubMed12937219

2003

TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinn-Yang Chen,Chin-Wen Chi,Hui-Ling Chen,Chiung-Pei Wan,Wu-Chang Yang,An-Hang Yang
View all publications

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