FITC Anti-GFP antibody

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord.

Detected using ab6662 and a standard FITC filter set. Axons are labeled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.

These images were kindly supplied as part of the review submitted by Andrew Toft.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

Immunofluorescence Microscopy using ab6662.

Tissue : Sf-1 : Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification).

Fixation : 4% PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution.

Antigen retrieval : Frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns.

Goat anti-GFP was used at 1/500 dilution in free floating imunnohistochemistry to detect GFP.

Secondary antibody : Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1 : 500 at 1/10,000 for 45 minutes at RT.

Localization : Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus.

Staining : eGFP as green fluorescent signal and sections were counterstained with DAPI.

• IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - FITC Anti-GFP antibody (AB6662)

Oscillation of Cyclin E and E2F target gene expression is deregulated in de2f1b mutant salivary glands.

Salivary glands of control and de2f1b mutant early (80–85 hr AEL) third instar larvae expressing PCNA-GFP (green, ab6662) are stained with anti-dE2F1 (red). The region where high PCNA-GFP is observed with low dE2F1 is marked by an asterisk.

For Immunostaining, third instar imaginal discs and salivary glands were dissected in PBS and immediately fixed in 4% formaldehyde in PBS for 20 minutes at room temperature with the exception of tissues subjected to anti-dE2F1 staining that were fixed for 30 minutes on ice. Fixed tissues were then washed with 0.3% PBST (0.3% TritonX-100 in 1XPBS) and 0.1% PBST (0.1% TritonX-100 in 1XPBS). Samples were incubated with appropriate amount of primary antibody in 0.1% PBST and 1%BSA overnight. Samples were then washed with 0.1% PBST, incubated in secondary antibody in 0.1% PBST and 1% BSA for 2 hours, followed by several washes in 0.1% PBST prior to mounting.

Kim at el PLoS Genet. 2018 Feb 8;14(2):e1007204. doi: 10.1371/journal.pgen.1007204. eCollection 2018 Feb. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Supplier Data

Western blot - FITC Anti-GFP antibody (AB6662)

Block : Blocking buffer for 30 minutes at RT.

All lanes:

Western blot - FITC Anti-GFP antibody (ab6662)

All lanes:

Western blot - Recombinant A. victoria GFP protein (Tag Free) (<a href='/en-us/products/proteins-peptides/recombinant-a-victoria-gfp-protein-ab84191'>ab84191</a>)

Secondary

All lanes:

Fluorescein goat secondary antibody for 60 minutes at RT at 1/1000 dilution

Predicted band size: 27 kDa

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