Mouse Monoclonal Glycophorin A antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GYPA.
Images
![Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (AB52452), expandable thumbnail](https://content.abcam.com/products/images/fitc-glycophorin-a-b-antibody-hir2-ab52452--flow-cytometry-img8033.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (AB52452)")
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Synthetic Peptide within Human GYPA. The exact immunogen used to generate this antibody is proprietary information. Database link P02724
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. |
Associated Products
Select an associated product type
3 products for Alternative Version
3 products for Alternative Product
Target data
Function
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
Alternative names
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GPA, GYPA
Recommended products
Mouse Monoclonal Glycophorin A antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GYPA.
Alternative names
Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, GPA, GYPA
Key facts
- Isotype
IgG2b
- Host species
Mouse
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS, 0.2% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Synthetic Peptide within Human GYPA. The exact immunogen used to generate this antibody is proprietary information. Database link P02724
- Clone number
HIR2
- Purification technique
Affinity purification Protein G
- Specificity
ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.
- Concentration
- Purification notes
Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
Glycophorin A and Glycophorin B are well-known sialoglycoproteins located on the surface of human erythrocytes. They are often referenced by alternate names like GYPHE for Glycophorin E and Glycophorin A+B for the combination of both proteins. Glycophorin A which can be labeled with FITC for detection has a molecular mass of approximately 31.3 kDa while Glycophorin B has a similar but slightly smaller mass. These proteins are essential components of the erythrocyte membrane contributing significantly to the negative charge and structural stability of red blood cells.
- Biological function summary
Glycophorins A and B interact with other proteins in the erythrocyte membrane influencing the cell's architecture and the stability of the cytoskeleton. These glycophorins associate with CD235a known for its role in red blood cell recognition and interactions. Glycophorin A participates in the formation of a protein complex with other membrane proteins contributing to the antigenic properties of red blood cells which is important in blood transfusion biology.
- Pathways
Glycophorin A and B participate in several key biological processes such as membrane organization and signal transduction pathways. They are involved in the MNS blood group system and interact with proteins like band 3 which is a critical component of the erythrocyte's anion exchange mechanism. This interaction helps facilitate ion transport and gas exchange in red blood cells.
- Associated diseases and disorders
Glycophorins A and B have associations with malaria and autoimmune hemolytic anemia. The malaria parasite Plasmodium falciparum interacts with these glycophorins to invade red blood cells. Changes in the glycan composition of glycophorin A contribute to the pathophysiology of certain autoimmune disorders potentially linking to immune recognition proteins. Managing these interactions and modifications can be important in designing therapeutic interventions for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452), expandable thumbnail](https://content.abcam.com/products/images/fitc-glycophorin-a-b-antibody-hir2-ab52452--flow-cytometry-img8033.jpg "Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)")
Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (ab52452)
Flow cytometry analysis (surface staining) of human peripheral blood labeling CD235a with ab52452.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com