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AB52452

FITC Anti-Glycophorin A + B antibody [HIR2]

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Mouse Monoclonal Glycophorin A antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GYPA.

View Alternative Names

CD235a, GPA, MNS, GYPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

1 Images
Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (AB52452)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Glycophorin A + B antibody [HIR2] (AB52452)

Flow cytometry analysis (surface staining) of human peripheral blood labeling CD235a with ab52452.

  • Unconjugated

    Anti-Glycophorin A + B antibody [HIR2]

  • 578 PE

    PE Anti-Glycophorin A + B antibody [HIR2]

  • Biotin

    Biotin Anti-Glycophorin A + B antibody [HIR2]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HIR2

Isotype

IgG2b

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human GYPA. The exact immunogen used to generate this antibody is proprietary information.

P02724

Specificity

ab52452 recognises N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins expressed on red blood cells and erythroid precursor cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>20 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }
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Product details

ab52452 agglutinates untreated RBCs but it failes to agglutinate papain-treated cells, binds to GPA, but weakly to GPB. The antibody is useful in erythroid cell development studies, because HIR2 antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells (mature erythrocytes are characteristically CD235a positive and CD45 and CD71 negative).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Prior to conjugation, ab52452 was purified by Protein G affinity chromatography. After conjugation, it was purified by site exclusion chromatography. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycophorin A and Glycophorin B are well-known sialoglycoproteins located on the surface of human erythrocytes. They are often referenced by alternate names like GYPHE for Glycophorin E and Glycophorin A+B for the combination of both proteins. Glycophorin A which can be labeled with FITC for detection has a molecular mass of approximately 31.3 kDa while Glycophorin B has a similar but slightly smaller mass. These proteins are essential components of the erythrocyte membrane contributing significantly to the negative charge and structural stability of red blood cells.
Biological function summary

Glycophorins A and B interact with other proteins in the erythrocyte membrane influencing the cell's architecture and the stability of the cytoskeleton. These glycophorins associate with CD235a known for its role in red blood cell recognition and interactions. Glycophorin A participates in the formation of a protein complex with other membrane proteins contributing to the antigenic properties of red blood cells which is important in blood transfusion biology.

Pathways

Glycophorin A and B participate in several key biological processes such as membrane organization and signal transduction pathways. They are involved in the MNS blood group system and interact with proteins like band 3 which is a critical component of the erythrocyte's anion exchange mechanism. This interaction helps facilitate ion transport and gas exchange in red blood cells.

Glycophorins A and B have associations with malaria and autoimmune hemolytic anemia. The malaria parasite Plasmodium falciparum interacts with these glycophorins to invade red blood cells. Changes in the glycan composition of glycophorin A contribute to the pathophysiology of certain autoimmune disorders potentially linking to immune recognition proteins. Managing these interactions and modifications can be important in designing therapeutic interventions for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed : 35835865). Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane.. (Microbial infection) Appears to be a receptor for Hepatitis A virus (HAV).. (Microbial infection) Receptor for P.falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans.
See full target information GYPA

Additional targets

GYPB
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Product promise

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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB26016

PE Anti-Glycophorin A + B antibody [HIR2]

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Alternative Version
Primary Antibodies

AB93548

Biotin Anti-Glycophorin A + B antibody [HIR2]

primary-antibodies

biotin-glycophorin-a-b-antibody-hir2-ab93548

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(1 reviews)

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