AB93561

FITC Anti-H2-Ea antibody [M5/114.15.2]

Name: Anti-H2-Ea FITC antibody [M5/114.15.2] (ab93561) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB93561

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(11 Publications)

Rat Monoclonal HA21 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 11 publications.

View Alternative Names

H2-IE-alpha, H2-Ea

1 Images

Flow Cytometry - FITC Anti-H2-Ea antibody [M5/114.15.2] (AB93561)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-H2-Ea antibody [M5/114.15.2] (AB93561)

Flow cytometric analysis of mouse C57BI/6 splenocytes labeling H2-Ea using ab93561 (0.25 μg) (Solid line) as compared to a FITC Rat IgG2b isotype control (0.25 μg) (Dashed line).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M5/114.15.2

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab93561 reacts with the mouse MHC Class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

H2-Ea also known as the I-A alpha chain is a protein with a molecular weight of approximately 33 kDa. It is a part of the major histocompatibility complex (MHC) class II molecules primarily expressed on the surface of antigen-presenting cells including dendritic cells macrophages and B cells. This protein provides a critical mechanical role in presenting peptide antigens to CD4+ T-helper cells facilitating immune responses.

Biological function summary

H2-Ea functions as a component of the MHC class II heterodimer complex participating in antigen processing and presentation. Upon forming a complex with other MHC class II molecules it binds peptides derived from extracellular proteins. This complex formation initiates immune recognition which is essential for the activation of CD4+ T cells. These cells then enable the immune system to tackle pathogens and coordinate the adaptive immune response efficiently.

Pathways

The immune recognition facilitated by H2-Ea is a pivotal step in the adaptive immune pathway. This protein interacts with other MHC molecules and proteins such as CD3 and CD4 during the antigen presentation process. In the pathway H2-Ea plays an important role in ensuring the adaptive immune system correctly activates and regulates responses against foreign antigens and potential threats to the body.

Alterations or deficiencies in H2-Ea expression can impact conditions such as autoimmune diseases. Aberrant H2-Ea function influences the pathogenesis of autoimmune diseases like Type 1 Diabetes and Rheumatoid Arthritis where immune regulation breaks down. The protein's interaction with autoantigens through MHC class II complexes is associated with disease mechanisms and progression often involving other immune-related proteins like Interleukin-6 (IL-6) and Tumor Necrosis Factor (TNF).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information H2-Ea

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed38931345

2024

Improved Immunotherapy Outcomes via Cuproptosis Upregulation of HLA-DRA Expression: Promoting the Aggregation of CD4 and CD8T Lymphocytes in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Wang,Yiwen Liu,Feng Xiong,Chunyang Wang

Cell host & microbe 32:1089-1102.e10 PubMed38889725

2024

H19 influenza A virus exhibits species-specific MHC class II receptor usage.

Applications

Unspecified application

Species

Unspecified reactive species

Umut Karakus,Ignacio Mena,Jithesh Kottur,Sara S El Zahed,Rocío Seoane,Soner Yildiz,Leanne Chen,Magdalena Plancarte,LeAnn Lindsay,Rebecca Halpin,Timothy B Stockwell,David E Wentworth,Geert-Jan Boons,Florian Krammer,Silke Stertz,Walter Boyce,Robert P de Vries,Aneel K Aggarwal,Adolfo García-Sastre

Molecular medicine reports 19:5377-5385 PubMed31059096

2019

Salmonella infection leads to severe intestinal inflammation and increased CD4+FoxP3+ Treg cells in streptozotocin‑induced hyperglycemic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shanlong Zhang,Meixiang Wang,Xuemei Wang,Helou Li,Hua Tang,Xiaojun Li

International journal of molecular medicine 39:1101-1110 PubMed28339016

2017

Paeonol ameliorates imiquimod-induced psoriasis-like skin lesions in BALB/c mice by inhibiting the maturation and activation of dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yujiao Meng,Mingxing Wang,Xiangjiang Xie,Tingting Di,Jingxia Zhao,Yan Lin,Xiaolong Xu,Ningfei Li,Yating Zhai,Yan Wang,Ping Li

Molecular medicine reports 12:2511-20 PubMed25936350

2015

Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Lin Zeng,Li-Hui Wang,Ping Li,Wei Wang,Jiong Yang

Proceedings of the National Academy of Sciences of the United States of America 104:234-9 PubMed17190803

2006

The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface.

Applications

Unspecified application

Species

Unspecified reactive species

Julia J Unternaehrer,Amy Chow,Marc Pypaert,Kayo Inaba,Ira Mellman

Infection and immunity 71:4137-43 PubMed12819105

2003

Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract.

Applications

Unspecified application

Species

Unspecified reactive species

Pascale Gueirard,Patrick Ave,Anne-Marie Balazuc,Sabine Thiberge,Michel Huerre,Genevieve Milon,Nicole Guiso

International immunology 14:1179-91 PubMed12356683

2002

Cooperative interaction of Ig(alpha) and Ig(beta) of the BCR regulates the kinetics and specificity of antigen targeting.

Applications

Species

Unspecified reactive species

Chang Li,Karyn Siemasko,Marcus R Clark,Wenxia Song

The American journal of pathology 160:141-9 PubMed11786408

2002

Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis).

Applications

Unspecified application

Species

Unspecified reactive species

Harini Bagavant,Colin Sharp,Barbara Kurth,Kenneth S K Tung

Immunology 89:238-44 PubMed8943720

1996

Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity.

Applications

Unspecified application

Species

Unspecified reactive species

S K Mendiratta,N Singh,V Bal,S Rath

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-H2-Ea antibody [M5/114.15.2]

Flow Cytometry - FITC Anti-H2-Ea antibody [M5/114.15.2] (AB93561)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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Product promise