AB1208

FITC Anti-HA tag antibody

Name: FITC conjugated Anti-HA tag antibody (ab1208) | Abcam
Brand: Abcam Limited
SKU: AB1208
Price: 585 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(6 Publications)

Anti-HA tag antibody - FITC conjugated (ab1208) is a rabbit polyclonal antibody detecting HA tag in Western Blot, ICC/IF, ELISA.

- Trusted since 2002

View Alternative Names

HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain

1 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-HA tag antibody (AB1208)

ICC/IF

AbReview20739****

Immunocytochemistry/ Immunofluorescence - FITC Anti-HA tag antibody (AB1208)

ab1208 staining Ha tag in human breast cancer cells (HA tagged expression plasmid) by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilised in 0.2% Triton X-100 in PBS and then blocked using 1% BSA and 3% FBS in PBS for 30 minutes at room temperature. Samples were then incubated with primary antibody at 1/50 for 1 hour.

Image courtesy of Dr Manikandan Periyasamy by Abreview.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Applications

ICC/IF, ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Product details

What is this antibody validated in?
FITC Anti-HA tag antibody (ab1208) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA. Tagged protein detection
HA tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

What is the molecular weight of HA tag?
Anti-HA tag FITC (ab1208) specifically detects a band for HA tag (UniProt: P03435) at a molecular weight of 1kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HA tag

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Protein science : a publication of the Protein Society 34:e70072 PubMed39981925

2025

Taming interleukin-12: Engineering of bispecific antibody-based IL-12 mimetics with biased agonism capacities.

Applications

Unspecified application

Species

Unspecified reactive species

Britta Lipinski,Laura Unmuth,Paul Arras,Ron Endruszeit,Stefan Becker,Jonathan Mathias Rödel,Jürgen Scheller,Silke Pudewell,Doreen M Floss,Simon Krah,Julia Harwardt,Achim Doerner,Laura Helming,Chunxiao Xu,Andreas Menrad,Andreas Evers,Harald Kolmar,Desislava Elter,Lukas Pekar,Stefan Zielonka

Nature communications 13:6020 PubMed36241646

2022

TLCD1 and TLCD2 regulate cellular phosphatidylethanolamine composition and promote the progression of non-alcoholic steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Kasparas Petkevicius,Henrik Palmgren,Matthew S Glover,Andrea Ahnmark,Anne-Christine Andréasson,Katja Madeyski-Bengtson,Hiroki Kawana,Erik L Allman,Delaney Kaper,Martin Uhrbom,Liselotte Andersson,Leif Aasehaug,Johan Forsström,Simonetta Wallin,Ingela Ahlstedt,Renata Leke,Daniel Karlsson,Hernán González-King,Lars Löfgren,Ralf Nilsson,Giovanni Pellegrini,Nozomu Kono,Junken Aoki,Sonja Hess,Grzegorz Sienski,Marc Pilon,Mohammad Bohlooly-Y,Marcello Maresca,Xiao-Rong Peng

ACS synthetic biology 11:2662-2671 PubMed35881049

2022

Engineering Adhesion to Target Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xavier Pierrat,Alix Pham,Jeremy P H Wong,Zainebe Al-Mayyah,Alexandre Persat

Molecular therapy. Methods & clinical development 17:409-417 PubMed32128346

2020

Factors Impacting Efficacy of AAV-Mediated CRISPR-Based Genome Editing for Treatment of Choroidal Neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Sook Hyun Chung,Iris Natalie Mollhoff,Uyen Nguyen,Amy Nguyen,Natalie Stucka,Eric Tieu,Suman Manna,Ratheesh Kumar Meleppat,Pengfei Zhang,Emerald Lovece Nguyen,Jared Fong,Robert Zawadzki,Glenn Yiu

Journal of virology 92: PubMed29093081

2018

Analysis of Select Herpes Simplex Virus 1 (HSV-1) Proteins for Restriction of Human Immunodeficiency Virus Type 1 (HIV-1): HSV-1 gM Protein Potently Restricts HIV-1 by Preventing Intracellular Transport and Processing of Env gp160.

Applications

Unspecified application

Species

Unspecified reactive species

Sachith Polpitiya Arachchige,Wyatt Henke,Ankita Pramanik,Maria Kalamvoki,Edward B Stephens

Proceedings of the National Academy of Sciences of 112:15654-9 PubMed26644563

2015

Tmem178 acts in a novel negative feedback loop targeting NFATc1 to regulate bone mass.

Applications

Unspecified application

Species

Unspecified reactive species

Corinne E Decker,Zhengfeng Yang,Ryan Rimer,Kyung-Hyun Park-Min,Claudia Macaubas,Elizabeth D Mellins,Deborah V Novack,Roberta Faccio

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-HA tag antibody

Immunocytochemistry/ Immunofluorescence - FITC Anti-HA tag antibody (AB1208)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (6)

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