AB69545

FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]

Name: FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (ab69545) | Abcam
Brand: Abcam Limited
SKU: AB69545
Rating: 4 (1 reviews)

(1 Review)

(6 Publications)

Mouse Monoclonal Heme Oxygenase 1 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Rat Hmox1.

View Alternative Names

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

1 Images

Flow Cytometry (Intracellular) - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB69545)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB69545)

Flow Cytometry of 10⁶ Jurkat cells stained using ab69545 at a concentration of 100 µg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HO-1-2

Isotype

IgG2b

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Native Full Length Protein corresponding to Rat Hmox1.

P06762

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2b-plpv219-isotype-control-ab91368'>ab91368</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 14^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 10: PubMed34762046

2021

The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Jonathan Lehrke,Michael Jeremy Shapiro,Matthew J Rajcula,Madeleine M Kennedy,Shaylene A McCue,Kay L Medina,Virginia Smith Shapiro

Frontiers in pharmacology 11:662 PubMed32508636

2020

Dihydroquercetin Activates AMPK/Nrf2/HO-1 Signaling in Macrophages and Attenuates Inflammation in LPS-Induced Endotoxemic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Lei,Yunfei Chai,Haoming Lin,Chunbo Chen,Mingyi Zhao,Weiping Xiong,Jian Zhuang,Xiaoping Fan

The Kaohsiung journal of medical sciences 36:741-749 PubMed32311231

2020

MiR-370 accelerated cerebral ischemia reperfusion injury via targeting SIRT6 and regulating Nrf2/ARE signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Fan Ruan,Ming Xie,Shu-Jia Gui,Fang Lan,Juan Wan,Yan Li

Journal of immunology (Baltimore, Md. : 1950) 190:6378-88 PubMed23650615

2013

Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Chiang,Masakazu Shinohara,Jesmond Dalli,Valbona Mirakaj,Megumi Kibi,Augustine M K Choi,Charles N Serhan

PLoS pathogens 8:e1002579 PubMed22438807

2012

HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children.

Applications

Flow Cyt

Species

Human

Michael Walther,Adam De Caul,Peter Aka,Madi Njie,Alfred Amambua-Ngwa,Brigitte Walther,Irene M Predazzi,Aubrey Cunnington,Susanne Deininger,Ebako N Takem,Augustine Ebonyi,Sebastian Weis,Robert Walton,Sarah Rowland-Jones,Giorgio Sirugo,Scott M Williams,David J Conway

Blood 117:4826-35 PubMed21389316

2011

The impact of inflammatory licensing on heme oxygenase-1-mediated induction of regulatory T cells by human mesenchymal stem cells.

Applications

Flow Cyt

Species

Human

Dimitrios Mougiakakos,Regina Jitschin,C Christian Johansson,Riki Okita,Rolf Kiessling,Katarina Le Blanc

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]

Flow Cytometry (Intracellular) - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB69545)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise