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AB20930

FITC Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

0

(1 Review)

|

(1 Publication)

Goat Polyclonal HBSAG antibody - conjugated to FITC. Suitable for ELISA and reacts with Hepatitis B virus samples. Cited in 1 publication.

View Alternative Names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Hepatitis B virus

Applications

ELISA

applications

Specificity

Purified surface antigen. Monospecific, reacts only with Hepatitis B virus.

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Reactivity data

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Product details

Covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Surface Antigen (HBsAg) also known as Hepatitis B surface antigen AD/AY serves as a significant marker for the Hepatitis B virus (HBV). This glycoprotein displays a molecular mass of approximately 24 kDa and consists of small medium and large protein forms. HBsAg is typically expressed in the infected liver cells and secreted into the bloodstream. This expression allows its use as a diagnostic marker for HBV infection through assays like HBsAg ELISA which detects the presence of the virus.
Biological function summary

The surface antigen plays an important role in the virus's attachment and entry into hepatocytes. HBsAg forms part of the viral envelope structure which facilitates the virus's ability to infect host cells. The presence of HBsAg is linked to an individual’s immune response specifically the production of hepatitis antibodies. Its interaction with the host’s immune system and antibodies like hep B antibodies are essential for understanding both viral pathogenesis and immunity to HBV infections.

Pathways

The Hepatitis B virus surface antigen plays critical parts in the infectious cycle and immune response pathways. One important pathway includes the endocytosis pathway where HBsAg is involved in the virus's entry into liver cells. The surface antigen also interacts with the human immune system influencing the expression of Hepatitis B antibodies. Connected proteins include those involved in the immune signaling where alterations affect the body's ability to mount an effective response against HBV.

The Hepatitis B Virus Surface Antigen is directly associated with Hepatitis B infection and its progression. Chronic HBV infection may lead to liver-related diseases such as cirrhosis and hepatocellular carcinoma. HBsAg presence serves as an indicator for diagnosing these liver diseases. The antigen's interaction with other viral proteins such as the core antigen and E antigen are important in the disease's progression and overall clinical outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.
See full target information S

Additional targets

S,S
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:682 PubMed29445209

2018

3D microfluidic liver cultures as a physiological preclinical tool for hepatitis B virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

A M Ortega-Prieto,J K Skelton,S N Wai,E Large,M Lussignol,G Vizcay-Barrena,D Hughes,R A Fleck,M Thursz,M T Catanese,M Dorner
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Product promise

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