AB32914

FITC Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

(1 Review)

(7 Publications)

Rabbit Polyclonal HBSAG antibody - conjugated to FITC. Suitable for ICC and reacts with Hepatitis B virus samples. Cited in 7 publications.

View Alternative Names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S, Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S, Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Hepatitis B virus

Applications

ICC

applications

Specificity

This antibody recognizes Hepatitis B Surface Antigen, subtypes ad and ay.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification notes

This antibody was purified to monospecific FITC.

Storage buffer

pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Surface Antigen (HBsAg) also known as Hepatitis B surface antigen AD/AY serves as a significant marker for the Hepatitis B virus (HBV). This glycoprotein displays a molecular mass of approximately 24 kDa and consists of small medium and large protein forms. HBsAg is typically expressed in the infected liver cells and secreted into the bloodstream. This expression allows its use as a diagnostic marker for HBV infection through assays like HBsAg ELISA which detects the presence of the virus.

Biological function summary

The surface antigen plays an important role in the virus's attachment and entry into hepatocytes. HBsAg forms part of the viral envelope structure which facilitates the virus's ability to infect host cells. The presence of HBsAg is linked to an individual’s immune response specifically the production of hepatitis antibodies. Its interaction with the host’s immune system and antibodies like hep B antibodies are essential for understanding both viral pathogenesis and immunity to HBV infections.

Pathways

The Hepatitis B virus surface antigen plays critical parts in the infectious cycle and immune response pathways. One important pathway includes the endocytosis pathway where HBsAg is involved in the virus's entry into liver cells. The surface antigen also interacts with the human immune system influencing the expression of Hepatitis B antibodies. Connected proteins include those involved in the immune signaling where alterations affect the body's ability to mount an effective response against HBV.

The Hepatitis B Virus Surface Antigen is directly associated with Hepatitis B infection and its progression. Chronic HBV infection may lead to liver-related diseases such as cirrhosis and hepatocellular carcinoma. HBsAg presence serves as an indicator for diagnosing these liver diseases. The antigen's interaction with other viral proteins such as the core antigen and E antigen are important in the disease's progression and overall clinical outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

See full target information S

Additional targets

S,S

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Antiviral research 226:105891 PubMed38649071

2024

Inhibition of Pim kinases triggers a broad antiviral activity by affecting innate immunity and via the PI3K-Akt-mTOR axis the endolysosomal system.

Applications

Unspecified application

Species

Unspecified reactive species

Mirco Glitscher,Nuka Ivalu Benz,Catarina Sabino,Robin Oliver Murra,Sascha Hein,Tobias Zahn,Ines Mhedhbi,Debora Stefanova,Daniela Bender,Sabine Werner,Eberhard Hildt

Journal of virology 98:e0042424 PubMed38629837

2024

The impact of HBx protein on mitochondrial dynamics and associated signaling pathways strongly depends on the hepatitis B virus genotype.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Schollmeier,Michael Basic,Mirco Glitscher,Eberhard Hildt

Nature communications 14:8440 PubMed38114531

2023

NDP52 mediates an antiviral response to hepatitis B virus infection through Rab9-dependent lysosomal degradation pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhi Cui,Tian Xia,Jianjin Zhao,Xiaoyu Ren,Tingtao Wu,Mireille Kameni,Xiaoju Guo,Li He,Jingao Guo,Aléria Duperray-Susini,Florence Levillayer,Jean-Marc Collard,Jin Zhong,Lifeng Pan,Frédéric Tangy,Pierre-Olivier Vidalain,Dongming Zhou,Yaming Jiu,Mathias Faure,Yu Wei

Cellular and molecular gastroenterology and hepatology 15:237-259 PubMed36184032

2022

Presence of Intact Hepatitis B Virions in Exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyan Wu,Mirco Glitscher,Susanne Tonnemacher,Anja Schollmeier,Jan Raupach,Tobias Zahn,Regina Eberle,Jacomine Krijnse-Locker,Michael Basic,Eberhard Hildt

Molecular therapy : the journal of the American Society of Gene Therapy 29:1794-1807 PubMed33484968

2021

The CD39 HBV surface protein-targeted CAR-T and personalized tumor-reactive CD8 T cells exhibit potent anti-HCC activity.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zou,Jizhou Tan,Ting Liu,Bingfeng Liu,Yaping Tang,Hui Zhang,Jiaping Li

Hepatology (Baltimore, Md.) 50:1380-91 PubMed19821533

2009

Hepatitis B virus as a gene delivery vector activating foreign antigenic T cell response that abrogates viral expression in mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Deng,Maryline Mancini-Bourgine,Xiaoming Zhang,Marie-Christine Cumont,Ren Zhu,Yu-Chun Lone,Marie-Louise Michel

Journal of virology 81:4244-54 PubMed17192308

2006

Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Deng,Jian-wei Zhai,Marie-Louise Michel,Jun Zhang,Jun Qin,Yu-ying Kong,Xin-xin Zhang,Agata Budkowska,Pierre Tiollais,Yuan Wang,You-hua Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (7)

Product promise