Skip to main content

Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HSPB1 phospho S82.

Be the first to review this product! Submit a review

Images

Flow Cytometry - FITC Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (AB278774), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 99.71% PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human HSPB1 phospho S82. The exact immunogen used to generate this antibody is proprietary information. Database link P04792

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
5 µL for 106 Cells
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Hsp27 phospho S82 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human HSPB1 phospho S82.

Key facts

Isotype
IgG
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human HSPB1 phospho S82. The exact immunogen used to generate this antibody is proprietary information. Database link P04792
Clone number
HSP27S82-CB2
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Associated diseases and disorders

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - FITC Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278774), expandable thumbnail

    Flow Cytometry - FITC Anti-Hsp27 (phospho S82) antibody [HSP27S82-CB2] (ab278774)

    Flow cytometric analysis of HeLa cells unstained and untreated cells as as negative control (blue) or untreated (red) or treated with UV and TPA (green) and stained using ab278774.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com