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AB322304

FITC Anti-ICAM1 antibody [EPR22161-284]

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Rabbit Recombinant Monoclonal ICAM1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD54, Icam-1, Icam1, Intercellular adhesion molecule 1, ICAM-1, MALA-2, MyD10

2 Images
Flow Cytometry (Intracellular) - FITC Anti-ICAM1 antibody [EPR22161-284] (AB322304)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-ICAM1 antibody [EPR22161-284] (AB322304)

Flow cytometry overlay histogram showing left Ramos positive cells and right negative HEK293 stained with ab322304 (red line). The cells were incubated in 1x PBS containing μg/ml human IgG and 10% normal goat serum followed by the antibody (ab322915) (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG [EPR25A] FITC used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Flow Cytometry - FITC Anti-ICAM1 antibody [EPR22161-284] (AB322304)
  • Flow Cyt

Lab

Flow Cytometry - FITC Anti-ICAM1 antibody [EPR22161-284] (AB322304)

Flow cytometry overlay histogram showing left A20 positive cells and right negative L-929 stained with ab322304 (red line). The cells were incubated in 1x PBS containing 10%; normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab322304) (1x 106 in 100ul at 0.2ug/ml (1/2500)) for 30min on ice.

Isotype control antibody (black line) was Rabbit IgG [EPR25A] FITC used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22161-284

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>" } } }
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AB174445

Human ICAM1 ELISA Kit (CD54)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
See full target information Icam1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com