JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275606

FITC Anti-IL33 antibody [03]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal IL-33 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL33 aa 100 to C-terminus.

View Alternative Names

C9orf26, IL1F11, NFHEV, IL33, Interleukin-33, IL-33, Interleukin-1 family member 11, Nuclear factor from high endothelial venules, IL-1F11, NF-HEV

1 Images
Flow Cytometry (Intracellular) - FITC Anti-IL33 antibody [03] (AB275606)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-IL33 antibody [03] (AB275606)

Intracellular flow cytometric analysis ofHUVEC (human umbilical vein endothelial cell line) cells labeling IL-33 with ab275606 (Black) compared with isotype control (Grey).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

03

Isotype

IgG2b

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human IL33 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95760

style
left-column

Complementary Products

Assay Kits

AB223865

Human IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-33 ELISA Kit (AB223865)

  • 0
  • 0
Primary Antibodies

AB84247

Biotin Anti-TSLP antibody

Sandwich ELISA - Biotin Anti-TSLP antibody (AB84247)

  • 0
  • 0
Assay Kits

AB223865

Human IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-33 ELISA Kit (AB223865)

  • 0
  • 0
Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1
Proteins & Peptides

AB281811

Recombinant Human IL-33 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human IL-33 protein (Active) (AB281811)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>10 μl/Test</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin 33 (IL-33) also known as IL1F11 or NF-HEV is a cytokine belonging to the IL-1 family. It is a protein with a molecular weight of approximately 30 kDa. IL-33 is mainly expressed in endothelial cells epithelial cells and fibroblasts. It exists in a full-length form in the nucleus and releases into the extracellular environment upon cellular damage or necrosis indicating its role as an alarmin. The expression of IL-33 can be upregulated in cells under stress or damaged conditions.
Biological function summary

In its role as a cytokine involved in immune response activation IL-33 functions as an alarmin signaling tissue damage or infection. It does not operate as part of a complex; instead IL-33 binds to the ST2 receptor (also known as IL1RL1) on target cells. This binding triggers the activity of immune cells such as type 2 helper T cells mast cells and innate lymphoid cells. By interacting with these immune cells IL-33 contributes to the body's inflammatory and repair processes controlling the early stages of immune response activation.

Pathways

IL-33 has a significant role in the signaling cascade related to the ST2/IL-1 receptor pathway. This pathway is essential for modulating inflammation and immune responses. Additionally IL-33 links with the NF-kB signaling pathway often involved in inflammatory responses. In these pathways its interactions with proteins like MyD88 and IRAK proteins are important for intracellular signaling and the promotion of cytokine production helping sustain the immune response.

IL-33 is associated with the pathogenesis of conditions such as asthma and rheumatoid arthritis. In asthma IL-33 contributes to airway inflammation and hypersensitivity acting in concert with other cytokines like IL-4 and IL-5 to amplify the type 2 immune response. In rheumatoid arthritis IL-33 engages with the ST2 receptor in joint tissues promoting inflammation and joint degradation. Such roles highlight IL-33's potential as a therapeutic target in modulating excessive immune responses in these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed : 16286016, PubMed : 19841166). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (PubMed : 17853410, PubMed : 18836528). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (PubMed : 17853410, PubMed : 18836528). Acts as an enhancer of polarization of alternatively activated macrophages (PubMed : 19841166). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (PubMed : 17853410, PubMed : 18836528). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed : 21734074). This form is rapidely lost upon angiogenic or pro-inflammatory activation (PubMed : 18787100).
See full target information IL33
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com