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AB95782

FITC Anti-IL3RA/CD123 antibody [6H6]

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(1 Publication)

Mouse Monoclonal IL3RA/CD123 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing IL3RA protein.

View Alternative Names

CD123, IL3R, IL3RA, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA

1 Images
Flow Cytometry - FITC Anti-IL3RA/CD123 antibody [6H6] (AB95782)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-IL3RA/CD123 antibody [6H6] (AB95782)

Staining of normal Human peripheral blood cells with anti-Human CD85g (ILT7) Alexa Fluor® 647 and 0.25 µg of Mouse IgG1k Isotype Control FITC (left) or ab95782 (right). Cells in the monocyte gate were used for analysis.

  • Unconjugated

    Anti-IL3RA/CD123 antibody [6H6]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-IL3RA/CD123 antibody [6H6]

  • 778 PE/Cy7®

    PE/Cy7® Anti-IL3RA/CD123 antibody [6H6]

  • 578 PE

    PE Anti-IL3RA/CD123 antibody [6H6]

  • Biotin

    Biotin Anti-IL3RA/CD123 antibody [6H6]

  • 660 APC

    APC Anti-IL3RA/CD123 antibody [6H6]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-IL3RA/CD123 antibody [6H6]

  • 667 PE/Cy5®

    PE/Cy5® Anti-IL3RA/CD123 antibody [6H6]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6H6

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing IL3RA protein. The exact immunogen used to generate this antibody is proprietary information.

P26951

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-kappa-mopc-21-isotype-control-ab106163'>ab106163</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: BSA, Gelatin, PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL3RA also known as CD123 or interleukin-3 receptor alpha chain is a transmembrane protein with a molecular weight of approximately 70-80 kDa. This protein frequently expresses on the surface of hematopoietic cells particularly those of myeloid lineage including mast cells and basophils. Researchers use CD123 as a marker for identifying certain cell types in flow cytometry. Antibodies such as anti-CD123 are available for various applications including identifying these cells in research and clinical settings.
Biological function summary

IL3RA forms part of a heterodimeric receptor complex binding with the beta-common (βc) chain to create the high-affinity IL-3 receptor. This complex plays a role in mediating the effects of interleukin-3 a cytokine that promotes the growth and differentiation of pluripotent hematopoietic stem cells. Through this mechanism the receptor influences immune response modulation particularly in allergic reactions and inflammation.

Pathways

IL3RA interacts with the JAK/STAT pathway which is essential for signal transduction and transcription activation in response to IL-3 binding. This pathway involves proteins like Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 5 (STAT5) which transmit signals from IL3RA engagement to the cell nucleus promoting gene expression associated with cell proliferation and survival.

IL3RA expression relates closely to certain hematological conditions such as acute myeloid leukemia (AML) and systemic mastocytosis. In AML increased expression of CD123 marks leukemic cells making it a target for therapeutic intervention. The interaction of IL3RA with FLT3 another tyrosine kinase is of particular interest in these disorders as both proteins contribute to the proliferation of malignant cells through related signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).
See full target information IL3RA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Hybridoma 19:455-61 PubMed11152397

2001

Characterization of monoclonal antibodies against mouse and rat platelet glycoprotein V (CD42d).

Applications

Unspecified application

Species

Unspecified reactive species

N Sato,N Kiyokawa,K Takada,M Itagaki,M Saito,T Sekino,T Suzuki,T Taguchi,K Mimori,F Lanza,J Fujimoto
View all publications
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