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AB210526

FITC Anti-Influenza A Virus Nucleoprotein antibody [D67J]

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(5 Publications)

Mouse Monoclonal NCAP antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Influenza A samples. Cited in 5 publications. Immunogen corresponding to Full Length Protein corresponding to Influenza A virus (A/Puerto Rico/8/1934(H1N1)) NP.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N, NP, Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein

1 Images
Flow Cytometry - FITC Anti-Influenza A Virus Nucleoprotein antibody [D67J] (AB210526)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Influenza A Virus Nucleoprotein antibody [D67J] (AB210526)

Flow cytometry analysis of 293T cells were infected Influenza PR8 virus labeling Influenza A Virus Nucleoprotein using ab210526 at 1/10, 1/20 and 1/40 dilutions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

D67J

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Influenza A

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Influenza A virus (A/Puerto Rico/8/1934(H1N1)) NP. The exact immunogen used to generate this antibody is proprietary information.

P03466

Specificity

This antibody reacts with Influenza A virus NP in viral and infected tissue samples.

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Influenza A Virus Nucleoprotein also known as NP or nucleoprotein influenza A plays a significant mechanical role in the virus life cycle. It is an essential structural component that encapsidates viral RNA forming ribonucleoprotein complexes important for replication and transcription. Influenza A nucleoprotein has a molecular weight of approximately 56 kDa and is expressed in infected host cells. Within the influenza virion the nucleoprotein interacts with the viral RNA and polymerase complex ensuring the integrity and functionality of the viral genome.
Biological function summary

The influenza A nucleoprotein is involved in regulating viral RNA synthesis and acts as a platform for the assembly of viral components. It does not directly form stable complexes with host proteins but it is central to the ribonucleoprotein complexes that are vital for the virus life cycle. This nucleoprotein assists in the virus's ability to hijack the host cellular machinery favoring its replication and infection processes. While the nucleoprotein itself does not form direct complexes with host proteins its interactions are significant for facilitating various stages of the viral life cycle.

Pathways

The influenza A nucleoprotein operates within the viral replication and transcription pathways. It associates with proteins such as PB1 PB2 and PA of the polymerase complex which is important for viral RNA synthesis. The nucleoprotein ensures the proper packaging of the viral genome to be recognized by the polymerase complex playing a pivotal role in the viral replication process. Through these pathways it indirectly impacts the host immune response as the virus must evade host defenses to ensure replication success.

The nucleoprotein influenza A is primarily linked to influenza infections a major respiratory illness. Influenza can manifest in a mild to severe form occasionally leading to pandemics. The nucleoprotein is an attractive target for antiviral drug development given its essential role in the viral life cycle. Moreover studies have shown that variations and mutations in the nucleoprotein can affect viral virulence and the outcome of infection. By understanding its mechanisms and interactions with other viral proteins like HA and NA researchers can better develop strategies to combat influenza outbreaks.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
See full target information NP

Additional targets

Influenza A Virus Nucleoprotein

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1056 PubMed41053808

2025

A novel anti-PD-L1/IL-8 bispecific antibody BP2402 enhances antitumor immunity and modulates inflammatory signaling in triple-negative breast cancer mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Song,Sumin Tang,Xuelei Pi,Yuanyuan Yan,Chenxi Hu,Liang Liu,Hongna Chen,Yating Zhang,Shishi Liu,Dan Yu,Chengkai Yin,Tianyan Liu,Xu Li,Deshan Li,Zhenzhong Wang,Wei Zhu,Kaiyuan Hui,Zhihang Liu,Xiaodong Jiang

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 204:114507 PubMed39303952

2024

Inhibition of influenza virus infection in mice by pulmonary administration of a spray dried antiviral drug.

Applications

Unspecified application

Species

Unspecified reactive species

Rick Heida,Paulo H Jacob Silva,Renate Akkerman,Jill Moser,Jacqueline de Vries-Idema,Aurélien Bornet,Sujeet Pawar,Francesco Stellacci,Henderik W Frijlink,Anke L W Huckriede,Wouter L J Hinrichs

Nature 623:820-827 PubMed37938771

2023

Targeting of intracellular oncoproteins with peptide-centric CARs.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Yarmarkovich,Quinlen F Marshall,John M Warrington,Rasika Premaratne,Alvin Farrel,David Groff,Wei Li,Moreno di Marco,Erin Runbeck,Hau Truong,Jugmohit S Toor,Sarvind Tripathi,Son Nguyen,Helena Shen,Tiffany Noel,Nicole L Church,Amber Weiner,Nathan Kendsersky,Dan Martinez,Rebecca Weisberg,Molly Christie,Laurence Eisenlohr,Kristopher R Bosse,Dimiter S Dimitrov,Stefan Stevanovic,Nikolaos G Sgourakis,Ben R Kiefel,John M Maris

International journal of molecular sciences 24: PubMed37569596

2023

Influenza A Infection Stimulates RIG-I and Enhances Effector Function of Primary Human NK Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adham Abuelola Mohamed,Sofía Soler,Julia Wegner,Eva Bartok,Sanda Stankovic,Andrew G Brooks,Martin Schlee

Nature 599:477-484 PubMed34732890

2021

Cross-HLA targeting of intracellular oncoproteins with peptide-centric CARs.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Yarmarkovich,Quinlen F Marshall,John M Warrington,Rasika Premaratne,Alvin Farrel,David Groff,Wei Li,Moreno di Marco,Erin Runbeck,Hau Truong,Jugmohit S Toor,Sarvind Tripathi,Son Nguyen,Helena Shen,Tiffany Noel,Nicole L Church,Amber Weiner,Nathan Kendsersky,Dan Martinez,Rebecca Weisberg,Molly Christie,Laurence Eisenlohr,Kristopher R Bosse,Dimiter S Dimitrov,Stefan Stevanovic,Nikolaos G Sgourakis,Ben R Kiefel,John M Maris
View all publications

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