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Knockout Tested Rabbit Recombinant Monoclonal Integrin alpha 2 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - FITC Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (AB322294), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/2500
Notes

-

Target data

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Alternative names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Integrin alpha 2 antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
FITC
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17338
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - FITC Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab322294), expandable thumbnail

    Flow Cytometry (Intracellular) - FITC Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (ab322294)

    Flow cytometry overlay histogram showing left wild-type HAP-1 positive cells and right negative ITGA2 knockout HAP-1 cells stained with ab322294 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10%; normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab322294) (1x 106 in 100ul at 0.2ug/ml (1/2500)) for 30min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG [EPR25A] FITC used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

    This antibody gave a positive signal in cells fixed with % methanol (5 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com