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AB326482

FITC Anti-Integrin alpha 4/CD49D antibody [9F10]

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Mouse Monoclonal Integrin alpha 4/CD49D antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD49d, CD49D, ITGA4, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha

2 Images
Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [9F10] (AB326482)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [9F10] (AB326482)

Separation of human CD49d positive lymphocytes (red-filled) from human blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab326482 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [9F10] (AB326482)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [9F10] (AB326482)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab326482 (4 μl reagent / 100 μl of peripheral whole blood).

  • 660 APC

    APC Anti-Integrin alpha 4/CD49D antibody [9F10]

  • 785 APC/Cy7®

    APC/Cy7® Anti-Integrin alpha 4/CD49D antibody [9F10]

  • 578 PE

    PE Anti-Integrin alpha 4/CD49D antibody [9F10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9F10

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).
See full target information ITGA4
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Product promise

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