AB30496

FITC Anti-Integrin alpha 6 antibody [450-30A]

Name: FITC Anti-Integrin alpha 6 antibody [450-30A] (ab30496) | Abcam
Brand: Abcam Limited
SKU: AB30496
Price: 260 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Integrin alpha 6 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ITGA6.

View Alternative Names

CD49f, Integrin alpha-6, CD49 antigen-like family member F, VLA-6, ITGA6

1 Images

Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (AB30496)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (AB30496)

Flow cytometric analysis of peripheral blood lymphocytes with 10μl for 10⁶ cells of anti human Integrin alpha 6 FITC conjugated (ab30496).

578 PE

PE Anti-Integrin alpha 6 antibody [450-30A]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

450-30A

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human ITGA6.

P23229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 6 also known as ITGA6 or CD49f functions as a transmembrane receptor protein involved in cell adhesion and signal transduction. As part of the broader integrin family integrin alpha 6 typically pairs with the beta 1 or beta 4 subunits forming the alpha6beta1 or alpha6beta4 integrins respectively. It has a molecular mass of approximately 150 kDa. This protein is mainly expressed in epithelial cells but also found in platelets certain immune cells and in the nervous system. Its expression patterns highlight its role in a variety of cellular and tissue functions.

Biological function summary

The integrin alpha 6 protein partners with the beta 4 subunit to form a heterodimer complex that plays an important role in cellular adhesion to the extracellular matrix specifically interacting with laminins. Its main action helps cells attach to the basement membrane which is an important component of epithelial tissue structure and integrity. The alpha 6 integrin provides structural cell support and participates in transmitting signals that influence cell behavior such as movement survival and proliferation.

Pathways

Integrins including the alpha 6 variant are important components in the integrin signaling pathway and are involved in cell motility and survival pathways. In particular the alpha 6 integrin partners with proteins like focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K) to initiate downstream signaling cascades. The interaction with laminins activates pathways critical for developmental processes and tissue remodeling highlighting their role in maintaining cellular environments.

Integrin alpha 6 has significant implications in cancer progression and skin blistering disorders. In various cancers such as breast and skin cancers upregulation of integrin alpha 6 promotes tumor growth and metastasis through interaction with proteins like collagen and laminins found in the tumor microenvironment. Furthermore in the context of blistering disorders such as epidermolysis bullosa mutations in the beta 4 subunit that pairs with alpha 6 can disrupt the integrity of epithelial tissues leading to severe impact on the skin’s structural stability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6 : ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-6/beta-4 (ITGA6 : ITGB4) is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGA6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of visualized experiments : JoVE : PubMed33586700

2021

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Yvon Woappi,Geraldine Ezeka,Justin Vercellino,Sean M Bloos,Kim E Creek,Lucia Pirisi

Stem cell research 49:102048 PubMed33128954

2020

Self-assembling 3D spheroid cultures of human neonatal keratinocytes have enhanced regenerative properties.

Applications

Unspecified application

Species

Unspecified reactive species

Yvon Woappi,Diego Altomare,Kim E Creek,Lucia Pirisi

The FEBS journal 284:2774-2785 PubMed28636114

2017

SET/I2PP2A overexpression induces phenotypic, molecular, and metabolic alterations in an oral keratinocyte cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Lays M Sobral,Ricardo D Coletta,Luciane C Alberici,Carlos Curti,Andréia M Leopoldino

Journal of periodontal research 45:703-8 PubMed20546107

2010

Magnetic separation and characterization of keratinocyte stem cells from human gingiva.

Applications

ICC/IF, Flow Cyt

Species

Human, Human

B Calenic,N Ishkitiev,K Yaegaki,T Imai,Y Kumazawa,M Nasu,T Hirata

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Integrin alpha 6 antibody [450-30A]

Flow Cytometry - FITC Anti-Integrin alpha 6 antibody [450-30A] (AB30496)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise