AB22486

FITC Anti-Integrin beta 4 antibody [450-9D]

Name: Anti-Integrin beta 4 FITC antibody [450-9D] (ab22486) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB22486
Price: 535 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal Integrin beta 4 antibody - conjugated to FITC. Suitable for IHC-Fr and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ITGB4.

View Alternative Names

CD104, Integrin beta-4, GP150, ITGB4

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

450-9D

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

Native Full Length Protein corresponding to Human ITGB4.

P16144

Epitope

the epitope is in the extracellular region of Integrin beta 4

Specificity

ab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 4 also known as ITGB4 integrin ß4 or integrine beta 4 is a transmembrane protein that mechanically links the extracellular matrix to the intracellular cytoskeleton. This protein has a molecular mass of approximately 205 kDa. Integrin beta 4 primarily expresses in epithelial cells particularly those forming stratified and transitional epithelia. It partners with alpha 6 integrin to form the alpha 6 beta 4 integrin complex which plays a significant role in the formation and maintenance of hemidesmosomes.

Biological function summary

Integrin beta 4 is important for anchoring epithelial cells to the basement membrane stabilizing cell layers in tissues. It interacts with intermediate filaments through specific cytoplasmic proteins. The integrin beta 4 complex ensures structural integrity essential for maintaining the skin and mucosal barriers. Integrations with proteins like M126 are essential for strengthening these interactions safeguarding cellular stability and promoting wound healing.

Pathways

Integrin beta 4 is embedded in key signaling pathways that influence cell adhesion and migration. It participates prominently in the PI3K/AKT and RAS pathway collaborations facilitating signal transduction essential for cell growth and survival. Through these pathways integrin beta 4 interacts with proteins like IS200BPIRG1A linking extracellular cues to internal cellular responses.

Integrin beta 4 has associations with epidermolysis bullosa and various cancers. In epidermolysis bullosa mutations in integrin beta 4 impair hemidesmosomal function leading to skin fragility. In cancer integrin beta 4 overexpression or mislocalization contributes to tumor progression affecting cell invasion and metastasis. Its interactions with IS200BPIAG1A or related pathways highlight the protein’s influence on carcinogenic processes and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6 : ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed : 20682778). ITGA6 : ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed : 22351760). ITGA6 : ITGB4 binds to IGF2 and this binding is essential for IGF2 signaling (PubMed : 28873464).

See full target information ITGB4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112346 PubMed40292313

2025

Deciphering pre-existing and induced 3D genome architecture changes involved in constricted melanoma migration.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Playter,Rosela Golloshi,Joshua H Garretson,Alvaro Rodriguez Gonzalez,Taiwo Habeeb Olajide,Ahmed Saad,Samuel John Benson,Rachel Patton McCord

Biology open 7: PubMed30026266

2018

Human trophoblasts are primarily distinguished from somatic cells by differences in the pattern rather than the degree of global CpG methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Teena K J B Gamage,William Schierding,Peter Tsai,Jackie L Ludgate,Lawrence W Chamley,Robert J Weeks,Erin C Macaulay,Joanna L James

Placenta 36:1412-8 PubMed26545962

2015

Differentiation of first trimester cytotrophoblast to extravillous trophoblast involves an epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia DaSilva-Arnold,Joanna L James,Abdulla Al-Khan,Stacy Zamudio,Nicholas P Illsley

Reproduction (Cambridge, England) 150:449-62 PubMed26248480

2015

Isolation and characterisation of a novel trophoblast side-population from first trimester placentae.

Applications

Unspecified application

Species

Unspecified reactive species

J L James,D G Hurley,T K J B Gamage,T Zhang,R Vather,P Pantham,P Murthi,L W Chamley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Integrin beta 4 antibody [450-9D]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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