AB27865

FITC Anti-Interferon gamma antibody [CC302]

Name: Anti-Interferon gamma FITC antibody [CC302] (ab27865) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB27865
Price: 525 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal Interferon gamma antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Pig, Human, Dog, Ferret, Horse, Cow, Goat, Sheep samples. Cited in 2 publications.

View Alternative Names

Interferon gamma, IFN-gamma, Immune interferon, IFNG

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CC302

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Sheep, Goat, Horse, Cow, Dog, Human, Pig, Ferret

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Cow": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Dog": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Ferret": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Goat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Horse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Pig": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." }, "Sheep": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 10μl of the suggested working dilution to label 106 cells in 100μl. Membrane permeabilisation is required for this application." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This antibody was purified by affinity chromatography from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.

Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

See full target information IFNG

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 10:2005 PubMed31497029

2019

Vaccination With Viral Vectors Expressing Chimeric Hemagglutinin, NP and M1 Antigens Protects Ferrets Against Influenza Virus Challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Meagan McMahon,Guha Asthagiri Arunkumar,Wen-Chun Liu,Daniel Stadlbauer,Randy A Albrecht,Vincent Pavot,Mario Aramouni,Teresa Lambe,Sarah C Gilbert,Florian Krammer

Infection and immunity 73:3778-82 PubMed15908411

2005

Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Reiko Nagata,Yoshihiro Muneta,Kazuhiro Yoshihara,Yuichi Yokomizo,Yasuyuki Mori

View all publications

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Product promise

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FITC Anti-Interferon gamma antibody [CC302]

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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