AB4208

FITC Anti-Kappa light chain antibody

Name: Anti-Kappa light chain FITC antibody (ab4208) Rabbit polyclonal IgG | Abcam
Brand: Abcam Limited
SKU: AB4208

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(1 Publication)

Rabbit Polyclonal Kappa light chain antibody - conjugated to FITC. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HCAK 1, IGKCD, Ig kappa chain C region, Immunoglobulin InV, Immunoglobulin KM, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Immunoglobulin kappa light chain constant region, Kappa 1 immunoglobulin light chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809, kappa light chain

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Kappa light chain antibody (AB4208)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Kappa light chain antibody (AB4208)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Kappa light chain with ab4208.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/40", "IHCP-species-notes": "<p>Pretreat tissue with Proteinase K for 30 minutes at 37°C. Incubate with primary antibody for 30-60 minutes at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Traces of contaminating antibodies have been removed by solid phase absorption. Only the kappa precipitation line appeared in cross immunoelectrophoresis.

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Kappa light chain

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40940788

2025

Prepubertal Diabetes Stagnates Testicular Development by Skewing Autophagy Homeostasis in Leydig Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zonghao Tang,Youkun Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Kappa light chain antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Kappa light chain antibody (AB4208)

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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