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AB278583

FITC Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

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(1 Publication)

Rabbit Recombinant Monoclonal KSR1 phospho S392 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KSR1 phospho S392.

View Alternative Names

KSR, KSR1, Kinase suppressor of Ras 1

1 Images
Flow Cytometry - FITC Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278583)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4] (AB278583)

Flow cytometric analysis of HeLa human adenocarcinoma cells untreated and unstained as negative control (blue) or treated with lambda phosphatase and stained (red) or untreated and stained (green) using ab278583.

  • Unconjugated

    Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

  • 578 PE

    PE Anti-KSR1 (phospho S392) antibody [KSR1S392-3A4]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

KSR1S392-3A4

Isotype

IgG

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human KSR1 phospho S392. The exact immunogen used to generate this antibody is proprietary information.

Q8IVT5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 99.71% PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KSR1 also known as Kinase Suppressor of Ras 1 serves as an important scaffold protein in cellular signaling. Weighing around 87 kDa KSR1 localizes in both the cytoplasm and the membrane of cells and shows expression in various tissues including the brain lung and ovaries. KSR1 does not have kinase activity itself but influences the activity of other kinases through its interactions.
Biological function summary

KSR1 exerts a regulatory role by organizing multi-protein complexes necessary for effective Ras and Raf signaling. This protein helps assemble the mitogen-activated protein kinase (MAPK) cascade complex which plays an essential role in cell growth and differentiation. As an important scaffolding component KSR1 enhances the efficiency of signal transduction pathways by facilitating the spatial organization of kinases involved in MAPK/ERK signaling.

Pathways

Signaling through KSR1 significantly impacts the MAPK/ERK and Ras pathways both of which are important for transmitting extracellular signals to the nucleus. KSR1 interacts with c-Raf MEK1/2 and ERK1/2 within these pathways forming a bridge that allows effective transduction of signals necessary for various cellular responses. By providing a platform for these kinases KSR1 ensures precise regulation of the signaling processes that control cell proliferation and survival.

Dysfunction in KSR1 activity associates with cancer and neurodegenerative disorders. KSR1 aberrations can contribute to oncogenic Ras-driven cancers due to its central role in controlling the Ras signaling pathway. Furthermore irregular KSR1 expression also links to neurodegenerative diseases through its impact on MAPK/ERK signaling. Proteins such as c-Raf and ERK1/2 which interact with KSR1 also play roles in these pathologies emphasizing the potential of KSR1 as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity). Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed : 29433126). Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity). Its kinase activity is unsure (By similarity). Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity).
See full target information KSR1 phospho S392
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of oncology 2021:4391581 PubMed34868313

2021

Baicalin Inhibits EMT through PDK1/AKT Signaling in Human Nonsmall Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Cheng-Bo Yuan,Bo Yang,Xuan Zhou
View all publications
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