AB77275

FITC Anti-Lambda Light chain antibody [4C2]

Name: FITC Anti-Lambda Light chain antibody [4C2] (ab77275) | Abcam
Brand: Abcam Limited
SKU: AB77275

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(1 Publication)

Anti-Lambda Light chain antibody [4C2] conjugated to FITC - suitable for flow cytometry, and reacts with human samples. Clone [4C2] is a mouse monoclonal antibody.

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3 Images

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Separation of human Ig lambda light chain positive B-lymphocytes (red-filled) from Ig lambda light chain negative CD19 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human Ig lambda light chain (4C2) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C2

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Goat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Guinea pig": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Hamster": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Rabbit": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "21 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." }, "Sheep": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Use 20μl for 106 cells or 100μl whole blood." } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

ab77275 is conjugated with Fluorescein isothiocyanate (FITC) under optimumconditions. The reagent is free of unconjugated FITC and adjusted for direct use.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lambda Light Chain also known as lambda chain or lambda protein forms a part of immunoglobulins (antibodies). Its mass typically ranges between 22 to 24 kDa. This protein is expressed primarily in B cells. Specifically it pairs with heavy chains to form the complete antibody molecule. It participates in the antigen recognition process supporting the immune system's defense mechanisms.

Biological function summary

Lambda Light Chain participates in immunological functions by contributing to the antigen-binding site of antibodies. It exists as a part of the immunoglobulin complex. The lambda chain alongside heavy chains forms the variable part of antibodies allowing specificity in antigen recognition. Its variation leads to a diverse antibody repertoire which is important for recognizing a wide range of pathogens.

Pathways

Lambda Light Chain fits into pathways involving adaptive immune responses. It is important in antigen processing and presentation pathways. In this context it interacts with the heavy chains which together drive the specificity and efficiency of immune response pathways. It also associates with proteins like the major histocompatibility complex (MHC) which facilitates antigen presentation to T cells.

Lambda Light Chain associates with multiple myeloma a cancer of plasma cells and light chain amyloidosis a disorder where light chains accumulate as amyloid fibrils in tissues. In these conditions proteins like immunoglobulin heavy chain and albumin often interact with or are affected by lambda chains providing insights into disease mechanisms and potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed : 24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGLV1-51

Alternative Names

Immunoglobulin lambda variable 1-51, Ig lambda chain V-I region BL2, Ig lambda chain V-I region EPS, Ig lambda chain V-I region NEW, Ig lambda chain V-I region NIG-64, IGLV1-51

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular neuroscience : MN 74:98 PubMed39414746

2024

Effects of Electroacupuncture at Varied Frequencies on Analgesia and Mechanisms in Sciatic Nerve Cuffing-Induced Neuropathic Pain Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kexin Fang,Wen Cheng,Bin Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-Lambda Light chain antibody [4C2]

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Flow Cytometry - FITC Anti-Lambda Light chain antibody [4C2] (AB77275)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise