AB24871

FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Name: FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker (ab24871) | Abcam
Brand: Abcam Limited
SKU: AB24871
Price: 270 USD
Availability: InStock

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(8 Publications)

Rat Monoclonal LAMP1 antibody - conjugated to FITC. Suitable for IP, EM, Flow Cyt, WB, IHC-Fr and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing Lamp1 protein.

View Alternative Names

CD107a, Lamp-1, Lamp1, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, 120 kDa lysosomal membrane glycoprotein, CD107 antigen-like family member A, LGP-120, Lysosomal membrane glycoprotein A, P2B, LGP-A

1 Images

Flow Cytometry (Intracellular) - FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB24871)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB24871)

Mouse MΦ cells were intracellularly stained with Rat Anti- Mouse CD107a-FITC (ab24871). The grey peak represents an isotype control. The purple peak represents the positive dataset.

Unconjugated

Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1D4B

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

EM, Flow Cyt, IP, WB, IHC-Fr

applications

Immunogen

Cell preparation containing Lamp1 protein. The exact immunogen used to generate this antibody is proprietary information.

P11438

Specificity

This antibody is specific to murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "EM" : {"fullname" : "Electron Microscopy", "shortname":"EM"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "EM-species-checked": "guaranteed", "EM-species-dilution-info": "", "EM-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/fitc-rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18446'>ab18446</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 15121881). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (By similarity). Also plays an important role in NK-cells cytotoxicity (PubMed : 23847195). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (By similarity). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (By similarity). Also implicated in tumor cell metastasis (PubMed : 2676155).

See full target information Lamp1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 18:30405-30420 PubMed39453865

2024

Mitochondrial Bioenergetics of Functional Wound Closure is Dependent on Macrophage-Keratinocyte Exosomal Crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Anu Sharma,Rajneesh Srivastava,Surya C Gnyawali,Pramod Bhasme,Adam J Anthony,Yi Xuan,Jonathan C Trinidad,Chandan K Sen,David E Clemmer,Sashwati Roy,Subhadip Ghatak

Science advances 9:eade8641 PubMed36724278

2023

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Triscott,Matthias Reist,Lukas Küng,Francielle C Mosele,Marika Lehner,John Gallon,Archna Ravi,Gurpreet K Arora,Simone de Brot,Mark Lundquist,Hector Gallart-Ayala,Julijana Ivanisevic,Salvatore Piscuoglio,Lewis C Cantley,Brooke M Emerling,Mark A Rubin

Cell host & microbe 29:1316-1332.e12 PubMed34237247

2021

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Philipp Walch,Joel Selkrig,Leigh A Knodler,Mandy Rettel,Frank Stein,Keith Fernandez,Cristina Viéitez,Clément M Potel,Karoline Scholzen,Matthias Geyer,Klemens Rottner,Olivia Steele-Mortimer,Mikhail M Savitski,David W Holden,Athanasios Typas

Scientific reports 11:10249 PubMed33986446

2021

Quantitative trait locus mapping identifies the Gpnmb gene as a modifier of mouse macrophage lysosome function.

Applications

Unspecified application

Species

Unspecified reactive species

Peggy Robinet,Brian Ritchey,Shuhui Wang Lorkowski,Alexander M Alzayed,Sophia DeGeorgia,Eve Schodowski,C Alicia Traughber,Jonathan D Smith

The Journal of biological chemistry 292:13774-13783 PubMed28705935

2017

Forkhead box O3 (FoxO3) regulates kidney tubular autophagy following urinary tract obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Li,Ronald Zviti,Catherine Ha,Zhao V Wang,Joseph A Hill,Fangming Lin

PloS one 8:e53239 PubMed23349704

2013

Absence of platelet phenotype in mice lacking the motor protein myosin Va.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew T Harper,Marion T J van den Bosch,Ingeborg Hers,Alastair W Poole

Osteoarthritis and cartilage 20:923-30 PubMed22554793

2012

Chondrocyte primary cilia shorten in response to osmotic challenge and are sites for endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

D R Rich,A L Clark

Journal of immunology (Baltimore, Md. : 1950) 187:4293-9 PubMed21911606

2011

Increased susceptibility of ST2-deficient mice to polymicrobial sepsis is associated with an impaired bactericidal function.

Applications

Flow Cyt

Species

Mouse

Julliette M Buckley,Jing Hua Liu,Chong Hui Li,Siobhan Blankson,Qiong Di Wu,Yong Jiang,H Paul Redmond,Jiang Huai Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Flow Cytometry (Intracellular) - FITC Anti-LAMP1 antibody [1D4B] - Lysosome Marker (AB24871)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise