JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275704

FITC Anti-LDL Receptor antibody [032]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal LDL Receptor antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Low-density lipoprotein receptor aa 1-800.

View Alternative Names

Low-density lipoprotein receptor, LDL receptor, Ldlr

1 Images
Flow Cytometry - FITC Anti-LDL Receptor antibody [032] (AB275704)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-LDL Receptor antibody [032] (AB275704)

Profile of anti-LDLR reactivity on RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with a mouse BD Fc Block purified anti-CD16/CD32 mAb 2.4G2 prior to staining, washed, then stained with ab275704 at 10 μl/Test.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

032

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Mouse Low-density lipoprotein receptor aa 1-800. The exact immunogen used to generate this antibody is proprietary information.

P35951

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>10 μl/Test.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LDL Receptor also called LDLR is a protein that plays an important role in the uptake and clearance of low-density lipoproteins (LDL) from the bloodstream. LDLR binds LDL particles facilitating their internalization through receptor-mediated endocytosis. This receptor is expressed mainly in the liver adrenal glands and other tissues involved in cholesterol metabolism. It has an approximate molecular weight of 160 kDa. Researchers often study LDLR using techniques like Western blotting to understand its presence and function.
Biological function summary

The LDL receptor interacts with LDL particles to regulate cholesterol levels in the body. It is part of a cell surface complex that recognizes and binds to apolipoprotein B-100 or apolipoprotein E present on LDL. This interaction initiates internalization of LDL leading to its degradation in lysosomes where cholesterol can be released and used by the cell. Mutations in the gene encoding LDLR can lead to inefficient cholesterol uptake influencing various metabolic processes.

Pathways

LDL receptor activities are integral to lipid metabolism and cholesterol homeostasis. Two important biological pathways that involve LDLR include the cholesterol biosynthesis pathway and the lipoprotein clearance pathway. Within these pathways LDLR collaborates closely with proteins like PCSK9 which modulates its expression and degradation and HMG-CoA reductase an important enzyme in cholesterol synthesis to balance cholesterol levels in the body.

Defects in the LDL receptor are strongly associated with familial hypercholesterolemia and atherosclerosis. These conditions arise from impaired clearance of LDL leading to elevated cholesterol levels which pose risks for cardiovascular diseases. LDLR dysfunctions are linked with the Protein PCSK9 whose gain-of-function mutations can exacerbate hypercholesterolemia by promoting degradation of LDLR while statins aim to increase LDLR expression to lower LDL cholesterol levels.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization.
See full target information Low-density lipoprotein receptor
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34830862

2021

Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Lin,Xiaolin Fang,Gehao Liang,Qing Luo,Yinghuan Cen,Yu Shi,Shijie Jia,Juanmei Li,Wenqian Yang,Andrew J Sanders,Chang Gong,Wenguo Jiang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB65390

Cholesterol Assay Kit - HDL and LDL/VLDL

Biochemical assay - Cholesterol Assay Kit - HDL and LDL/VLDL (AB65390)

  • 4
  • 10
Primary Antibodies

AB223339

FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Recombinant

Flow Cytometry (Intracellular) - FITC Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB223339)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB185194

Anti-PCSK9 antibody [EPR17827-117]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PCSK9 antibody [EPR17827-117] (AB185194)

  • 0
  • 0
Proteins & Peptides

AB167759

Recombinant mouse PCSK9 protein

Bioactive

Functional Studies - Recombinant mouse PCSK9 protein (AB167759)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com