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AB275723

FITC Anti-LIMPII antibody [052]

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(1 Publication)

Rabbit Recombinant Monoclonal LIMPII antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Scarb2 aa 1-450.

View Alternative Names

Lysosome membrane protein 2, 85 kDa lysosomal membrane sialoglycoprotein, Lysosome membrane protein II, Scavenger receptor class B member 2, LGP85, LIMP II, Scarb2

1 Images
Flow Cytometry (Intracellular) - FITC Anti-LIMPII antibody [052] (AB275723)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-LIMPII antibody [052] (AB275723)

Intracellular flow cytometric analysis of LIMPII expression in mouse spleen lymphocytes. The cells were treated according to manufacturers manual, and then stained with ab275723 at 10μl/Test. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

052

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Mouse Scarb2 aa 1-450. The exact immunogen used to generate this antibody is proprietary information.

O35114

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>10 μl/Test.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LIMPII also known as LIMP-2 is a lysosomal membrane protein involved in the transport and degradation processes within cells. It has an approximate molecular mass of 54 kDa. LIMPII is mainly expressed in the lysosomal membranes of a variety of tissues notably in the kidney liver and spleen. Its structural characteristics enable it to function effectively within these cellular organelles delivering enzymes and other molecules essential for cellular maintenance and function.
Biological function summary

The protein plays a critical role in lipid and protein degradation and helps maintain cellular homeostasis. As part of the lysosomal membrane LIMPII facilitates the uptake and processing of large biomolecules into the lysosome. It functions as a receptor for the uptake of β-glucocerebrosidase forming a complex to assist in its proper delivery from the endoplasmic reticulum to the lysosomes. This receptor-ligand relationship ensures efficient enzymatic activity necessary for lipid metabolism.

Pathways

One finds that LIMPII participates in the sphingolipid metabolism pathway and is vital for glucosylceramide catabolism. It interacts with glucocerebrosidase in this pathway which plays a significant role in the metabolism of glycosphingolipids. Another pathway where LIMPII has importance is the autophagy pathway where it contributes to the fusion of autophagosomes with lysosomes. Its activity supports the turnover of cellular components maintaining cellular metabolic balance.

LIMPII's dysfunction has links to Gaucher's disease and atherosclerosis. Both conditions relate to improper lipid metabolism. In Gaucher's disease the interaction between LIMPII and glucocerebrosidase becomes disrupted leading to lysosomal storage issues. In contrast altered expressions of LIMPII correlate with atherosclerosis possibly due to its role in lipid processing. These associations suggest that LIMPII could be a significant factor in therapeutic approaches for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a lysosomal receptor for glucosylceramidase (GBA1) targeting.
See full target information Scarb2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34830862

2021

Silencing CTNND1 Mediates Triple-Negative Breast Cancer Bone Metastasis via Upregulating CXCR4/CXCL12 Axis and Neutrophils Infiltration in Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Lin,Xiaolin Fang,Gehao Liang,Qing Luo,Yinghuan Cen,Yu Shi,Shijie Jia,Juanmei Li,Wenqian Yang,Andrew J Sanders,Chang Gong,Wenguo Jiang
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Product promise

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