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AB81710

FITC Anti-LOX 1 antibody [23C11]

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(5 Publications)

Mouse Monoclonal LOX 1 antibody - conjugated to FITC. Suitable for Flow Cyt, FuncS (Neut/Block), ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OLR1 aa 50 to C-terminus.

View Alternative Names

CLEC8A, LOX1, OLR1, Oxidized low-density lipoprotein receptor 1, Ox-LDL receptor 1, C-type lectin domain family 8 member A, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, LOX-1, Lectin-like oxLDL receptor 1, hLOX-1

2 Images
Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (AB81710)
  • Flow Cyt

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Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (AB81710)

Overlay histogram showing A549 cells stained with ab81710 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81710, 0.5μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (AB81710)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-LOX 1 antibody [23C11] (AB81710)

Flow cytometric analysis showing expression of LOX1 on human PBMC cells at 10 μg/mL concentration.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

23C11

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

FuncS (Neut/Block), ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human OLR1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P78380

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>Antibody totally prevented Hsp70 binding to LOX-1-CHO but not mock-transfected CHO cells.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FuncSNeutBlock-species-checked": "predicted", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab81710 neutralizes the biological activity of LOX 1. It also inhibits Hsp70 binding to dendritic cells and Hsp70 induced antigen cross representation. In vivo, targeting LOX1 with a tumour antigen using anti LOX 1 antibody ab81710 induces anti tumour immunity.
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Properties and storage information

Form
Liquid
Purification notes
Purified, 0.2 µm filtered solution.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.. (Microbial infection) May serve as a receptor for adhesin A variant 3 (nadA) of N.meningitidis.
See full target information OLR1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oncoimmunology 12:2203073 PubMed37089448

2023

Expression of gene on tumor-associated macrophages of head and neck squamous cell carcinoma, and its correlation with clinical outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Yan Zhao,Xin Xia,Song Mei,Yixuan Huang,Yingying Zhu,Shuting Yu,Xingming Chen

Journal of translational medicine 21:121 PubMed36788538

2023

Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangdong Li,Ziyi Wang,Chenyu Jiao,Yu Zhang,Nan Xia,Wenjie Yu,Xuejiao Chen,Likalamu Pascalia Wikana,Yue Liu,Linfeng Sun,Minhao Chen,Yuhao Xiao,Yuhua Shi,Sheng Han,Liyong Pu

Journal of virology 82:3466-79 PubMed18216094

2008

Scavenger receptor class B is required for hepatitis C virus uptake and cross-presentation by human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Heidi Barth,Eva K Schnober,Christoph Neumann-Haefelin,Christine Thumann,Mirjam B Zeisel,Helmut M Diepolder,Zongyi Hu,T Jake Liang,Hubert E Blum,Robert Thimme,Mélanie Lambotin,Thomas F Baumert

Immunity 22:551-60 PubMed15894273

2005

Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors.

Applications

ICC/IF, Flow Cyt

Species

Human, Human

Pascale Jeannin,Barbara Bottazzi,Marina Sironi,Andrea Doni,Marco Rusnati,Marco Presta,Virginia Maina,Giovanni Magistrelli,Jean François Haeuw,Guillaume Hoeffel,Nathalie Thieblemont,Nathalie Corvaia,Cecilia Garlanda,Yves Delneste,Alberto Mantovani

Immunity 17:353-62 PubMed12354387

2002

Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation.

Applications

Unspecified application

Species

Human

Yves Delneste,Giovanni Magistrelli,Jean Gauchat,Jean Haeuw,Jean Aubry,Kayo Nakamura,Naoko Kawakami-Honda,Liliane Goetsch,Tatsuya Sawamura,Jean Bonnefoy,Pascale Jeannin
View all publications
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