AB25024

FITC Anti-Ly6g antibody [RB6-8C5]

Name: Anti-Ly6g FITC antibody [RB6-8C5] (ab25024) Rat monoclonal IgG2b | Abcam
Brand: Abcam Limited
SKU: AB25024
Price: 605 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(19 Publications)

Rat Monoclonal Ly6g antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 19 publications.

View Alternative Names

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1, Ly6g

4 Images

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cyt

AbReview27588****

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

ab25024 at a 1/100 dilution detecting Ly6g (Gr1) in murine blood cells by Flow Cytometry. Gating strategy was for monocytes.

Image courtesy of an anonymous Abreview.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 at 0.5μg.

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling Ly6g with ab25024 0.5μg (right panel), Rat IgG2b (FITC) isotype control at 0.5μg (left panel).

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

BALB/c bone marrow cells were stained with either rat IgG2b-FITC (as an isotype control) or rat anti-mouse Ly-6G-FITC (ab25024) at a concentration of 1ug/10⁶ cells. Large cells were then gated and analyzed on a flow cytometer.

Unconjugated

Anti-Ly6g + Ly6c antibody [RB6-8C5]
redFluor™ 710

redFluor™ 710 Anti-Ly6g antibody [RB6-8C5]
578 PE

PE Anti-Ly6g antibody [RB6-8C5]
660 APC

APC Anti-Ly6g antibody [RB6-8C5]
778 PE/Cy7®

PE/Cy7® Anti-Ly6g antibody [RB6-8C5]
450 violetFluor™ 450

violetFluor™ 450 Anti-Ly6g antibody [RB6-8C5]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RB6-8C5

Isotype

IgG2b

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Specificity

ab25024 recognises mouse Mouse Ly-6G.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ly6G also known as lymphocyte antigen 6 complex locus G6D 1A8 or 8C5 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein. The molecule has a molecular weight of about 25-30 kDa. Its expression is mainly on neutrophils and a subpopulation of eosinophils in mice. It plays a role in the innate immune system marking these immune cells for differentiation and response to infection. Researchers consider it a specific marker for detecting and isolating mouse neutrophils often using methods such as Ly6G staining or anti-Ly6G antibodies for identification.

Biological function summary

Ly6G functions in the immune response by facilitating the migration and adhesion of neutrophils to the site of infection or injury. It is part of a protein complex on the cell surface that includes other Ly6 proteins. This group of proteins helps to modulate signal transduction for immune cell functions. Ly6G serves as an anchor through its GPI linkage aiding cell-to-cell communication and signal amplification in inflammation processes.

Pathways

Ly6G is significantly integrated into the immune signaling pathways notably the toll-like receptor (TLR) signaling pathway. Through these pathways Ly6G influences neutrophil migration and activation. Closely associated proteins include CD11b and CD18 which interact with Ly6G in neutrophil functions including adhesion and transmigration. These pathways are essential for orchestrating the body's defense mechanisms against pathogens.

Ly6G plays an essential role in inflammatory conditions and autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of Ly6G can alter neutrophil behavior which contributes to disease pathology. Researchers are investigating the relationship between Ly6G and other immune-related proteins like myeloperoxidase (MPO) and elastase as these protein interactions may impact the development and progression of inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ly6g

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 16:841733 PubMed35281296

2022

Sympathetic Nerve-Mediated Fellow Eye Pain During Sequential Cataract Surgery by Regulating Granulocyte Colony Stimulating Factor CSF3.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Fan,Caixia Fan,Benxiang Qi,Bin Zhang,Wenfeng Li,Xia Qi,Xiaomin Liu,Bi Ning Zhang,Yusen Huang

Medicina (Kaunas, Lithuania) 57: PubMed33921654

2021

The p38/MK2 Axis in Monocytes of Fibromyalgia Syndrome Patients: An Explorative Study.

Applications

Unspecified application

Species

Unspecified reactive species

Boya Nugraha,Renate Scheibe,Christoph Korallus,Matthias Gaestel,Christoph Gutenbrunner

Oncology letters 21:286 PubMed33732362

2021

Curcumin inhibits the growth of liver cancer by impairing myeloid-derived suppressor cells in murine tumor tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Tian,Liu Liao,Qing Zhou,Xiaodi Huang,Piao Zheng,Yinmei Guo,Tianhao Deng,Xuefei Tian

Frontiers in medicine 7:577578 PubMed33425934

2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomal miR-25 Regulates the Ubiquitination and Degradation of Runx2 by SMURF1 to Promote Fracture Healing in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yikun Jiang,Jun Zhang,Zhengwei Li,Guoliang Jia

Stem cell research & therapy 11:455 PubMed33109266

2020

microRNA-375 released from extracellular vesicles of bone marrow mesenchymal stem cells exerts anti-oncogenic effects against cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Ding,Jinhua Liu,Xiaofei Zhang

Experimental cell research 394:112101 PubMed32474064

2020

A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Lv,Yiming Xu,Hongqiang Cheng,Yuehai Ke,Xue Zhang,Kejing Ying

Frontiers in immunology 10:2088 PubMed31552036

2019

IL-1β Blockade Attenuates Thrombosis in a Neutrophil Extracellular Trap-Dependent Breast Cancer Model.

Applications

Unspecified application

Species

Unspecified reactive species

Tainá Gomes,Carolina B S Várady,André L Lourenço,Daniella M Mizurini,Araci M R Rondon,Ana C Leal,Barbara S Gonçalves,Dumith Chequer Bou-Habib,Emiliano Medei,Robson Q Monteiro

Immunity 51:258-271.e5 PubMed31350176

2019

The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew S Kimball,Frank M Davis,Aaron denDekker,Amrita D Joshi,Matthew A Schaller,Jennifer Bermick,Xianying Xing,Charles F Burant,Andrea T Obi,Dylan Nysz,Scott Robinson,Ron Allen,Nicholas W Lukacs,Peter K Henke,Johann E Gudjonsson,Bethany B Moore,Steve L Kunkel,Katherine A Gallagher

International journal of biological sciences 15:1618-1629 PubMed31360105

2019

Tripartite Motif 8 Deficiency Relieves Hepatic Ischaemia/reperfusion Injury via TAK1-dependent Signalling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu Tao,Wang Tianyu,Zhou Jiangqiao,Chen Zhongbao,Ma Xiaoxiong,Zhang Long,Zou Jilin

Investigative ophthalmology & visual science 60:1298-1308 PubMed30933257

2019

Mycoplasma Ocular Infection in Subretinal Graft Transplantation of iPS Cells-Derived Retinal Pigment Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichi Makabe,Sunao Sugita,Ayumi Hono,Hiroyuki Kamao,Masayo Takahashi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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violetfluor-450-ly6g-antibody-rb6-8c5-ab253070

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Ly6g antibody [RB6-8C5]

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Flow Cytometry - FITC Anti-Ly6g antibody [RB6-8C5] (AB25024)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

Product promise

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