AB58841

FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker

Name: Anti-M6PR (cation independent) FITC antibody [MEM-238] (ab58841) Mouse monoclonal IgG1 | Abcam
Brand: Abcam Limited
SKU: AB58841
Price: 275 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Mouse Monoclonal M6PR (cation independent) antibody - conjugated to FITC. Suitable for Flow Cyt, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Virus within Human IGF2R.

View Alternative Names

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

2 Images

Flow Cytometry (Intracellular) - FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB58841)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB58841)

Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1μg/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (1μg/1x10⁶ cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB58841)

Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB58841)

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-238) FITC antibody (ab58841) (red, concentration in sample 3 μg/ml) and mouse IgG1 isotype control (MOPC-21) FITC antibody (blue, concentration in sample 3 μg/ml).

Unconjugated

Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker
Biotin

Biotin Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker
578 PE

PE Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-238

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, Flow Cyt (Intra)

applications

Immunogen

Recombinant Virus within Human IGF2R. The exact immunogen used to generate this antibody is proprietary information.

P11717

Epitope

ab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "8.3 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cation-independent mannose 6-phosphate receptor (M6PR) is an important player in cellular transport mechanisms. Also known as the insulin-like growth factor 2 receptor (IGF2R) this protein weighs approximately 300 kDa. M6PR is present on the membrane of lysosomes and works as a lysosome membrane marker. It functions as a sorting receptor in the Golgi apparatus and endosomes facilitating the transport of lysosomal enzymes from the Golgi to lysosomes and back for function and degradation.

Biological function summary

The M6PR enables essential cellular processes by mediating the trafficking of enzymes important to lysosomal function. This receptor takes part in recognizing and binding proteins tagged with mannose 6-phosphate signals allowing their transport to lysosomes. IGF2R engages in forming transient complexes with these enzymes within the cellular trafficking circuitry therefore ensuring the proper delivery of enzyme cargo to its target destination.

Pathways

M6PR fits importantly within lysosomal enzyme transport and the insulin-like growth factor (IGF) pathway. It partially regulates the homeostasis of IGF2 by facilitating its degradation modulating cell proliferation growth and development. Steric interactions with IGF2 and other proteins like mannose receptor homologs and IGF-binding proteins outline its influence within these pathways highlighting its multifunctionality.

M6PR connects to certain cancers and lysosomal storage disorders. Dysfunctional pathway interactions or mutations within IGF2R often relate with types of cancer such as breast and liver cancers due to its influence on IGF2 regulation and cell proliferation. Additionally it associates with lysosomal storage diseases as lysosome-related pathologies emerge from impaired enzyme transport reflecting the M6PR's intricate role in cellular homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed : 18817523, PubMed : 2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed : 18817523, PubMed : 2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed : 18817523). This receptor also binds IGF2 (PubMed : 18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed : 10900005).

See full target information IGF2R

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Anticancer research 29:1383-8 PubMed19414391

2009

Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression.

Applications

Inhib

Species

Human

Friedemann Laube

The Journal of biological chemistry 277:40575-82 PubMed12189157

2002

The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.

Applications

WB, Flow Cyt

Species

Human, Human

Vladimír Leksa,Samuel Godár,Marek Cebecauer,Ivan Hilgert,Johannes Breuss,Ulrich H Weidle,Václav Horejsí,Bernd R Binder,Hannes Stockinger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker

Flow Cytometry (Intracellular) - FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB58841)

Flow Cytometry - FITC Anti-M6PR (cation independent) antibody [MEM-238] - Lysosome Membrane Marker (AB58841)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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