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AB25028

FITC Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

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(1 Publication)

Mouse Monoclonal HA11 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing MHC Class 1 H2 Db/H2-D1 protein.

View Alternative Names

H-2D(B), H2-D1

1 Images
Flow Cytometry - FITC Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25028)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25028)

Mouse C57BL/6 splenocytes stained for MHC Class 1 H2 Db/H2-D1 using ab25028 at 1 μg/106 cells in flow cytometric analysis.

  • Unconjugated

    Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

  • 578 PE

    PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

27-11-13

Isotype

IgG2a

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

An epitope on the a chain of H-2Db

Specificity

ab25028 recognizes with the a3 domain of H-2Db class I MHC (H2-D1) antigen. The antibody cross-reacts with the a3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.

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Alternative Products

Primary Antibodies

AB25547

PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]

Flow Cytometry - PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (AB25547)

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Primary Antibodies

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Primary Antibodies

AB170867

Anti-MHC Class II beta antibody [EPR11227]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-ab18455'>ab18455</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MHC Class I H2-D1 also known as H2-Db is an important protein within the major histocompatibility complex. This protein carries a molecular mass of approximately 45 kDa. H2-Db is expressed on the surface of almost all nucleated cells. As a protein it plays an important role in immune response by presenting peptide antigens to cytotoxic T cells. Other similar proteins like H2-Kb and H2-Kd are also involved in antigen presentation though each has distinct peptide binding preferences.
Biological function summary

The MHC Class I H2-Db interacts with peptides derived from cytosolic proteins and loads them onto its structure. It forms a complex with beta-2 microglobulin and presents these peptides on the cell surface. This action is essential in signaling to CD8+ T cells which scan for non-self peptides originating from infections or cellular abnormalities. The method by which H2-Db binds peptides ensures precise immune surveillance.

Pathways

H2-Db operates prominently in the antigen processing and presentation pathway. This pathway plays an important role in immune response and involves proteases that generate peptide fragments. H2-Db interacts with transporter associated with antigen processing (TAP) proteins to bind peptides enabling effective display of antigens. Furthermore its activity connects with other immune proteins such as H2-Kb in presenting a comprehensive range of antigens to immune cells.

The involvement of H2-Db in autoimmune diseases and viral infections is significant. Certain autoimmune conditions arise when self-antigens are mistakenly presented triggering an immune response. Infections like viral hepatitis show how pathogens can manipulate H2-Db to evade immune detection. Tumor cells may also exploit H2-Db's antigen-presenting function. Proteins such as H2-Kb may interact similarly highlighting the need for MHC Class I diversity in combating disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the presentation of foreign antigens to the immune system.
See full target information H2-D1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 190:418-27 PubMed23209324

2012

TLR3, TRIF, and caspase 8 determine double-stranded RNA-induced epithelial cell death and survival in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher S McAllister,Omar Lakhdari,Guillaume Pineton de Chambrun,Mélanie G Gareau,Alexis Broquet,Gin Hyug Lee,Steven Shenouda,Lars Eckmann,Martin F Kagnoff
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Product promise

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