AB106163

FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control

Name: FITC Mouse IgG1, monoclonal - Isotype control (ab106163) | Abcam
Brand: Abcam Limited
SKU: AB106163
Price: 145 USD
Availability: InStock

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(13 Publications)

FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control Cited in 13 publications.

View Alternative Names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

Unconjugated

Mouse IgG1, kappa monoclonal [MOPC-21] - isotype control
660 APC

APC Mouse IgG1 kappa [MOPC-21] - Isotype Control
665 Alexa Fluor® 647

Alexa Fluor® 647 Mouse IgG1 kappa [MOPC-21] - Isotype Control
Biotin

Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control
695 PerCP/Cy5.5®

PerCP/Cy5.5® Mouse IgG1, Kappa Monoclonal [MOPC-21] - Isotype Control
785 APC/Cy7®

APC/Cy7® Mouse IgG1 kappa [MOPC-21] - Isotype Control
778 PE/Cy7®

PE/Cy7® Mouse IgG1 kappa Isotype Control

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MOPC-21

Isotype

IgG1

Light chain type

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Applications

IHC-P, WB, IP, Flow Cyt, IHC-Fr

applications

Specificity

ab106163 (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues.

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Product details

ab106163 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg per ml of incubation mixture), change the conditions of your experiment to reduce the background.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

> 95% (by SDS-PAGE)

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.

See full target information IGHG4

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 16: PubMed38931935

2024

In Vivo Evaluation of Ga-Labeled NOTA-EGFRvIII Aptamer in EGFRvIII-Positive Glioblastoma Xenografted Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Young Park,Ye Lim Cho,Tae Sup Lee,Daekyun Lee,Ju-Hyung Kang,Soryong Lim,Yujin Lee,Jae Hyun Lim,Won Jun Kang

Photochemistry and photobiology 100:1419-1430 PubMed38234287

2024

Evaluating the effect of strontium ranelate and photobiomodulation on cementogenic and osteogenic differentiation of buccal fat pad-derived stem cells: An in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

S Mohaghegh,H Fathi,F Molaasadollah,M Teimoori,N Chiniforush,N Taghipour,F Shekarchi,H Nokhbatolfoghahaei

Diabetes 71:2597-2611 PubMed36125850

2022

Critical Role for Macrophages in the Developmental Programming of Pancreatic β-Cell Area in Offspring of Hypertensive Pregnancies.

Applications

Unspecified application

Species

Unspecified reactive species

Kate M Root,Brian Akhaphong,Melissa A Cedars,Alexa M Molin,Margaretta E Huchthausen,Connor F Laule,Ronald R Regal,Emilyn U Alejandro,Jean F Regal

Journal of innate immunity 14:629-642 PubMed35613551

2022

Regulation of TLR10 Expression and Its Role in Chemotaxis of Human Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Yadu Balachandran,Sarah Caldwell,Gurpreet Kaur Aulakh,Baljit Singh

Journal of cellular and molecular medicine 24:13853-13862 PubMed33124775

2020

The stiffness-controlled release of interleukin-6 by cardiac fibroblasts is dependent on integrin α2β1.

Applications

Unspecified application

Species

Unspecified reactive species

Małgorzata Gałdyszyńska,Justyna Bobrowska,Małgorzata Lekka,Paulina Radwańska,Lucyna Piera,Jacek Szymański,Jacek Drobnik

Scientific reports 9:13142 PubMed31511575

2019

Interplay of Plasmodium falciparum and thrombin in brain endothelial barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Avril,Max Benjamin,Mary-Margaret Dols,Joseph D Smith

The Journal of pathology 248:155-163 PubMed30680725

2019

Hepatitis C virus infection induces endoplasmic reticulum stress and apoptosis in human fetal liver stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Guo,Wei-Li Liu,Dong Yang,Zhi-Qiang Shen,Zhi-Gang Qiu,Min Jin,Jun-Wen Li

Biomedicine & pharmacotherapy = Biomedecine & phar 103:517-523 PubMed29677537

2018

The rescue effect of mesenchymal stem cell on sodium iodate-induced retinal pigment epithelial cell death through deactivation of NF-κB-mediated NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiying Mao,Ting Pan,Han Shen,Huiyu Xi,Songtao Yuan,Qinghuai Liu

Toxicological sciences : an official journal of the Society of Toxicology 156:275-288 PubMed28115644

2017

Editor's Highlight: Multiparametric Image Analysis of Rat Dorsal Root Ganglion Cultures to Evaluate Peripheral Neuropathy-Inducing Chemotherapeutics.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Guo,John Hamre,Sandy Eldridge,Holger P Behrsing,Facundo M Cutuli,Jodie Mussio,Myrtle Davis

Blood 111:5037-46 PubMed18337560

2008

IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Smed-Sörensen,Markus Moll,Tan-Yun Cheng,Karin Loré,Anna-Carin Norlin,Leif Perbeck,D Branch Moody,Anna-Lena Spetz,Johan K Sandberg

View all publications

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Product promise

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FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (13)

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