FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control Cited in 8 publications.
IgG1
Mouse
FITC
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IP | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Expected | Dilution info - | Notes - |
Select an associated product type
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control Cited in 8 publications.
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
IgG1
Mouse
FITC
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS
Liquid
Monoclonal
MOPC-21
Affinity purification Protein G
ab106163 (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed Rat and Human tissues.
kappa
> 95% (by SDS-PAGE)
Blue Ice
+4°C
Store in the dark
ab106163 is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
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