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AB314952

FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

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FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control Suitable for Flow Cyt (Intra) and reacts with samples.
1 Images
Flow Cytometry (Intracellular) - FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB314952)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control (AB314952)

Flow cytometry overlay histogram showing HeLa cells stained with ab119688 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 90% methanol for 30 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab119688) (1x 106 in 100μl at 1.0μg/ml) for 30min at 22°C.

Isotype control antibody (black line) was ab314952 (1.0μg/ml (1/500)) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

  • 578 PE

    PE Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

  • Unconjugated

    Mouse IgG1 monoclonal [R312-MouseIgG1]-Isotype control

  • Carrier free

    Mouse IgG1 monoclonal [R312-MouseIgG1] - Isotype control - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

R312-MouseIgG1

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

Flow Cyt (Intra)

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mouse IgG1 also known as immunoglobulin G1 is an important antibody isotype in mice. It has a molecular mass of approximately 150 kDa. Mouse IgG1 is found in the serum and extracellular fluids where it helps to mediate immune functions. This antibody is produced by plasma cells which originate from B lymphocytes. Its structure is comprised of two heavy chains and two light chains forming a Y-shaped molecule that facilitates antigen binding.
Biological function summary

Immunoglobulin G1 plays a significant role in adaptive immunity as it can engage with different cellular receptors and complement proteins to mediate immune responses. It does not function as part of a complex by itself but rather interacts with other immune system components to execute its tasks. Mouse IgG1 is known for its ability to neutralize pathogens agglutinate invading microbes and opsonize them for enhanced phagocytosis by immune cells like macrophages and neutrophils.

Pathways

The signaling processes associated with mouse IgG1 involve pathways such as the Fc gamma receptor pathway and the classical complement pathway. Engagement of its Fc region with Fc gamma receptors on the surface of immune cells leads to effector functions like phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). In these pathways proteins like complement component C1q and other Fc gamma receptor subtypes play key roles in modulating the immune responses initiated by IgG1.

Immunoglobulin G1 is relevant in conditions like autoimmune diseases and allergic reactions where inappropriate immune activation or regulation can be observed. In autoimmune diseases for example IgG1 can form immune complexes that deposit in tissues and activate inflammatory cascades involving proteins like complement factors and other immunoglobulin subclasses. Its involvement in allergic responses often involves IgG1 competing with IgE antibodies for antigen binding altering the allergic response dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com