AB20962

FITC Anti-Mycobacterium tuberculosis antibody

Name: FITC Anti-Mycobacterium tuberculosis antibody (ab20962) | Abcam
Brand: Abcam Limited
SKU: AB20962
Rating: 2 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal Mycobacterium tuberculosis antibody - conjugated to FITC. Suitable for IHC-P, ICC/IF and reacts with Mycobacterium tuberculosis samples. Cited in 7 publications.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mycobacterium tuberculosis

Applications

ICC/IF, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Acetone fixation is recommended." }, "Mycobacterium tuberculosis": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Acetone fixation is recommended." }, "Salmonella typhi": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "Acetone fixation is recommended." } } }

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Product details

The antibody is covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mycobacterium tuberculosis sometimes referred to as M. tuberculosis is a pathogenic bacterial species in the Mycobacterium genus. It is the causative agent of tuberculosis (TB) in humans and has a relatively large cellular mass characterized by a thick and complex cell wall rich in mycolic acids. This bacterium expresses in the lungs where it primarily resides in pulmonary macrophages after inhalation. The robust cell wall serves as a barrier against phagocytosis and contributes to its resistance to many antimicrobial agents.

Biological function summary

The bacteria stimulate a host immune response causing the formation of granulomas which are small nodular aggregations of immune cells. Mycobacterium tuberculosis employs several mechanisms to inhibit the lysosomal degradation processes in macrophages. It is part of a complex interplay involving the immune system which tries to control its spread. This interaction allows it to persist in a dormant state within the host making it difficult to eradicate completely and able to reactivate later.

Pathways

Mycobacterium tuberculosis's survival and pathogenicity involve several critical biological processes. The bacteria actively dodge destruction through pathways that inhibit host cell apoptosis and modulate cytokine production. Specific proteins like the 6-kda early secretory antigen target (ESAT-6) and culture filtrate protein 10 (CFP-10) complexes play a vital role in these mechanisms manipulating host immune signaling pathways to facilitate prolonged infection.

Mycobacterium tuberculosis is directly associated with tuberculosis a disease that can affect different body organs but most often targets the lungs. The pathogen’s persistence mechanisms implicate it in chronic infections and proteins like Ag85 complex interact with host defenses to further complicate control of the disease. Another disorder linked to its infection is latent TB where the bacteria remain inactive but viable within the host posing ongoing public health challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Mycobacterium tuberculosis

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013267 PubMed40523023

2025

APOE protects against severe infection with Mycobacterium tuberculosis by restraining production of neutrophil extracellular traps.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Liu,Dat Mai,Ana N Jahn,Tara A Murray,John D Aitchison,Benjamin H Gern,Kevin B Urdahl,Alan Aderem,Alan H Diercks,Elizabeth S Gold

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 30:376-396 PubMed35384137

2022

Foamy macrophages potentially inhibit tuberculous wound healing by inhibiting the TLRs/NF-κB signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Bin Yin,Zexin Zhang,Shuting Mao,Wu Bao,Wenqin Lian,Yueying Fan,Chao Hong,Yingjun Su,Chiyu Jia

PLoS pathogens 17:e1009643 PubMed34166469

2021

Rifampicin exposure reveals within-host Mycobacterium tuberculosis diversity in patients with delayed culture conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Genestet,Elisabeth Hodille,Alexia Barbry,Jean-Luc Berland,Jonathan Hoffmann,Emilie Westeel,Fabiola Bastian,Michel Guichardant,Samuel Venner,Gérard Lina,Christophe Ginevra,Florence Ader,Sylvain Goutelle,Oana Dumitrescu

ACS infectious diseases 5:1996-2002 PubMed31345032

2019

Radiosynthesis and PET Bioimaging of Br-Bedaquiline in a Murine Model of Tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alvaro A Ordonez,Laurence S Carroll,Sudhanshu Abhishek,Filipa Mota,Camilo A Ruiz-Bedoya,Mariah H Klunk,Alok K Singh,Joel S Freundlich,Ronnie C Mease,Sanjay K Jain

Nucleic acids research 44:10571-10587 PubMed27604870

2016

Alu repeats as transcriptional regulatory platforms in macrophage responses to M. tuberculosis infection.

Applications

Unspecified application

Species

Human

Manuella Bouttier,David Laperriere,Babak Memari,Joseph Mangiapane,Amanda Fiore,Eric Mitchell,Mark Verway,Marcel A Behr,Robert Sladek,Luis B Barreiro,Sylvie Mader,John H White

PLoS pathogens 9:e1003407 PubMed23762029

2013

Vitamin D induces interleukin-1β expression: paracrine macrophage epithelial signaling controls M. tuberculosis infection.

Applications

ICC/IF

Species

Mouse

Mark Verway,Manuella Bouttier,Tian-Tian Wang,Marilyn Carrier,Mario Calderon,Beum-Soo An,Emmanuelle Devemy,Fiona McIntosh,Maziar Divangahi,Marcel A Behr,John H White

Journal of bacteriology 191:6584-91 PubMed19717592

2009

Mycobacterium tuberculosis is able to accumulate and utilize cholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Brzostek,Jakub Pawelczyk,Anna Rumijowska-Galewicz,Bozena Dziadek,Jaroslaw Dziadek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Mycobacterium tuberculosis antibody

Key facts

Host species

Clonality

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise