AB90812

FITC Anti-Myeloperoxidase antibody [2D4]

Name: FITC conjugated Anti-Myeloperoxidase antibody [2D4] (ab90812) | Abcam
Brand: Abcam Limited
SKU: AB90812
Price: 485 USD
Availability: InStock

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(33 Publications)

Anti-Myeloperoxidase antibody [2D4] - FITC conjugated (ab90812) is a mouse monoclonal antibody detecting Myeloperoxidase in Flow Cytometry (Intra), Flow Cytometry. Suitable for Mouse, Rat.

- Over 20 publications
- Trusted since 2010

View Alternative Names

Myeloperoxidase, MPO

1 Images

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2D4] (AB90812)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2D4] (AB90812)

Detection of MPO in Wehi3BD+ cells. Red, black and blue line represent the isotype control, cells only and ab90812 at 20 μg/ml, respectively.

Unconjugated

Anti-Myeloperoxidase antibody [2D4]
Biotin

Biotin Anti-Myeloperoxidase antibody [2D4]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D4

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse, Rat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
FITC Anti-Myeloperoxidase antibody [2D4] (ab90812) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Mouse, Rat samples.

Trusted by the scientific community
Anti-Myeloperoxidase [2D4] FITC (ab90812) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.

Related products
Antibody clone 2D4 is also available pre-conjugated to a variety of labels for your convenience – Anti-Myeloperoxidase FITC [2D4] (ab90812).

Other related products
We have a range of other formats of antibody clone [2D4] also available for your convenience: ab90810, FITC - ab90812

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Properties and storage information

Form

Liquid

Purification notes

ab90812 is 0.2 µm filtered

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:96 PubMed38308301

2024

NLRP3 exacerbates EAE severity through ROS-dependent NET formation in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Da Jeong Byun,Jaeho Lee,Kyungryung Ko,Young-Min Hyun

Nature immunology 24:925-940 PubMed37188941

2023

Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Serena Gullotta,Donatella De Feo,Ekaterina Friebel,Aurora Semerano,Giulia Maria Scotti,Andrea Bergamaschi,Erica Butti,Elena Brambilla,Angela Genchi,Alessia Capotondo,Mattia Gallizioli,Simona Coviello,Marco Piccoli,Tiziana Vigo,Patrizia Della Valle,Paola Ronchi,Giancarlo Comi,Armando D'Angelo,Norma Maugeri,Luisa Roveri,Antonio Uccelli,Burkhard Becher,Gianvito Martino,Marco Bacigaluppi

Immune network 23:e27 PubMed37416934

2023

NLRP3 Exacerbate NETosis-Associated Neuroinflammation in an LPS-Induced Inflamed Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Da Jeong Byun,Jaeho Lee,Je-Wook Yu,Young-Min Hyun

Cancer cell 41:356-372.e10 PubMed36706760

2023

Neutrophil-activating therapy for the treatment of cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ian L Linde,Tyler R Prestwood,Jingtao Qiu,Genay Pilarowski,Miles H Linde,Xiangyue Zhang,Lei Shen,Nathan E Reticker-Flynn,David Kung-Chun Chiu,Lauren Y Sheu,Simon Van Deursen,Lorna L Tolentino,Wen-Chao Song,Edgar G Engleman

JCI insight 7: PubMed36346670

2022

NKG2D receptor activation drives primary graft dysfunction severity and poor lung transplantation outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel R Calabrese,Tasha Tsao,Mélia Magnen,Colin Valet,Ying Gao,Beñat Mallavia,Jennifer J Tian,Emily A Aminian,Kristin M Wang,Avishai Shemesh,Elman B Punzalan,Aartik Sarma,Carolyn S Calfee,Stephanie A Christenson,Charles R Langelier,Steven R Hays,Jeffrey A Golden,Lorriana E Leard,Mary Ellen Kleinhenz,Nicholas A Kolaitis,Rupal Shah,Aida Venado,Lewis L Lanier,John R Greenland,David M Sayah,Abbas Ardehali,Jasleen Kukreja,S Samuel Weigt,John A Belperio,Jonathan P Singer,Mark R Looney

Frontiers in pharmacology 13:1054176 PubMed36467039

2022

Xuebijing injection inhibited neutrophil extracellular traps to reverse lung injury in sepsis mice reducing Gasdermin D.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Shang,Zhi-Sen Zhang,Xin-Tong Wang,Jing Chang,Meng-En Zhou,Ming Lyu,Shuang He,Jian Yang,Yan-Xu Chang,Yuefei Wang,Ming-Chun Li,Xiumei Gao,Yan Zhu,Yuxin Feng

Oxidative medicine and cellular longevity 2022:8217663 PubMed35178161

2022

MPO/HOCl Facilitates Apoptosis and Ferroptosis in the SOD1 Motor Neuron of Amyotrophic Lateral Sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jialing Peng,Jingrui Pan,Jingjing Mo,Ying Peng

Frontiers in immunology 12:780210 PubMed35003095

2021

Extracellular CIRP Induces Macrophage Extracellular Trap Formation Gasdermin D Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchan Lee,Bridgette Reilly,Chuyi Tan,Ping Wang,Monowar Aziz

Advanced healthcare materials 11:e2102439 PubMed34859964

2021

Encountering and Wrestling: Neutrophils Recognize and Defensively Degrade Graphene Oxide.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyi Huang,Sijie Li,Yanlei Liu,Behafarid Ghalandari,Ling Hao,Chengjie Huang,Wenqiong Su,Yuqing Ke,Daxiang Cui,Xiao Zhi,Xianting Ding

Cells 10: PubMed34943852

2021

Repeated Social Defeat Exaggerates Fibrin-Rich Clot Formation by Enhancing Neutrophil Extracellular Trap Formation via Platelet-Neutrophil Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Sugimoto,Hiroyuki Yamada,Naotoshi Wada,Shinichiro Motoyama,Makoto Saburi,Hiroshi Kubota,Daisuke Miyawaki,Noriyuki Wakana,Daisuke Kami,Takehiro Ogata,Masakazu Ibi,Satoaki Matoba

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Myeloperoxidase antibody [2D4]

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2D4] (AB90812)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (33)

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