AB90812

FITC Anti-Myeloperoxidase antibody [2D4]

Name: FITC Anti-Myeloperoxidase antibody [2D4] (ab90812) | Abcam
Brand: Abcam Limited
SKU: AB90812
Price: 490 USD
Availability: InStock

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(42 Publications )

Anti-Myeloperoxidase antibody [2D4] conjugated to FITC - suitable for intracellular flow cytometry, and reacts with mouse, rat samples. Clone [2D4] is a mouse monoclonal antibody cited in 14 publications.

1 Images

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2D4] (AB90812)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2D4] (AB90812)

Detection of MPO in Wehi3BD+ cells. Red, black and blue line represent the isotype control, cells only and ab90812 at 20 μg/ml, respectively.

Unconjugated

Anti-Myeloperoxidase antibody [2D4]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D4

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse, Rat

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
FITC Anti-Myeloperoxidase antibody [2D4] (ab90812) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Mouse, Rat samples.

Trusted by the scientific community
Anti-Myeloperoxidase [2D4] FITC (ab90812) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.

Related products
Antibody clone 2D4 is also available pre-conjugated to a variety of labels for your convenience – Anti-Myeloperoxidase FITC [2D4] (ab90812).

Other related products
We have a range of other formats of antibody clone [2D4] also available for your convenience: ab90810, FITC - ab90812

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Properties and storage information

Form

Liquid

Purification notes

ab90812 is 0.2 µm filtered

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 11593004). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low density lipoprotein particles and limits vascular damage (By similarity).

See full target information Mpo

Alternative Names

Myeloperoxidase, MPO, Mpo

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Publications (42)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1608428 PubMed40821824

2025

Molecular and subcellular mechanisms of vital macrophage extracellular trap formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchan Lee,Max Brenner,Monowar Aziz,Ping Wang

International journal of molecular medicine 56: PubMed40747675

2025

High‑dose X‑ray irradiation induces NETosis via the eCIRP/TREM‑1 axis in mouse neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Yamaga,Atsushi Murao,Monowar Aziz,Ping Wang,Max Brenner

International journal of molecular medicine 56: PubMed40709382

2025

Dulaglutide markedly prevents peritoneal fibrosis in a rodent model of chronic kidney disease: Insights into the pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chao Yang,Ya Yue,Yi-Ting Wang,John Y Chiang,Ben-Chung Cheng,Tsuen-Wei Hsu,Yi-Ling Chen,Yi-Chen Li,Hon-Kan Yip

Inflammation 48:2756-2771 PubMed39725843

2024

Rhaponticin Alleviates Collagen-induced Arthritis by Inhibiting NLRP3/GSDMD-mediated Neutrophil Extracellular Traps.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zhang,Xinyue Xie,Qinhao Shen,Chenchen Yuan,Guotao Lu,Weiming Xiao,Weijuan Gong,Xiaoyan Fu,Xuebing Feng

iScience 27:111284 PubMed39618498

2024

induced trained immunity in macrophages confers heterologous protection against gram-negative bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Simon R Carlile,Seán C Cahill,Eóin C O'Brien,Nuno G B Neto,Michael G Monaghan,Rachel M McLoughlin

Cell death discovery 10:395 PubMed39237520

2024

Highly sensitive magnetic particle imaging of abdominal aortic aneurysm NETosis with anti-Ly6G iron oxide nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Wang,Ruijing Zhang,Xiaohua Jia,Siqi Gao,Tingting Gao,Keyi Fan,Yaling Li,Shule Wang,Maolin Qiao,Sheng Yan,Hui Hui,Honglin Dong

Journal of neuroinflammation 21:70 PubMed38515139

2024

Hypochlorous acid derived from microglial myeloperoxidase could mediate high-mobility group box 1 release from neurons to amplify brain damage in cerebral ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Chen,Jingrui Pan,Zhe Gong,Meiling Wu,Xiaoni Zhang,Hansen Chen,Dan Yang,Suhua Qi,Ying Peng,Jiangang Shen

Journal of leukocyte biology 116:632-643 PubMed38484156

2024

B-1a cells scavenge NETs to attenuate sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Kensuke Murata,Atsushi Murao,Chuyi Tan,Ping Wang,Monowar Aziz

Cell communication and signaling : CCS 22:96 PubMed38308301

2024

NLRP3 exacerbates EAE severity through ROS-dependent NET formation in the mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Da Jeong Byun,Jaeho Lee,Kyungryung Ko,Young-Min Hyun

Nature immunology 24:925-940 PubMed37188941

2023

Age-induced alterations of granulopoiesis generate atypical neutrophils that aggravate stroke pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgia Serena Gullotta,Donatella De Feo,Ekaterina Friebel,Aurora Semerano,Giulia Maria Scotti,Andrea Bergamaschi,Erica Butti,Elena Brambilla,Angela Genchi,Alessia Capotondo,Mattia Gallizioli,Simona Coviello,Marco Piccoli,Tiziana Vigo,Patrizia Della Valle,Paola Ronchi,Giancarlo Comi,Armando D'Angelo,Norma Maugeri,Luisa Roveri,Antonio Uccelli,Burkhard Becher,Gianvito Martino,Marco Bacigaluppi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Myeloperoxidase antibody [2D4]

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [2D4] (AB90812)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (42)

Product promise