AB269284

FITC Anti-Myeloperoxidase antibody [MPO421-8B2]

Name: Anti-Myeloperoxidase FITC antibody [MPO421-8B2] (ab269284) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB269284
Price: 530 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Myeloperoxidase antibody - conjugated to FITC. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human MPO.

View Alternative Names

Myeloperoxidase, MPO

3 Images

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Intracellular Flow Cytometry analysis (intracellular staining) of human peripheral blood labeling Myeloperoxidase with ab269284.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using ab269284 (4 ul reagent/100 ul of peripheral whole blood).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using ab269284 (4 ul reagent / 100 ul of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MPO421-8B2

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Full Length Protein corresponding to Human MPO. The exact immunogen used to generate this antibody is proprietary information.

P05164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "4 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p>Or 100 μL of whole blood.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108957 PubMed38333692

2024

EP300 restores the glycolytic activity and anti-tumor function of CD8 cytotoxic T cells in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiu Xia,Xiaoxu Ding,Chao Ji,Dabo Zhou,Xun Sun,Tiancong Liu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-Myeloperoxidase antibody [MPO421-8B2]

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Flow Cytometry (Intracellular) - FITC Anti-Myeloperoxidase antibody [MPO421-8B2] (AB269284)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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