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AB69763

FITC Anti-NCAM1 antibody [MEM-188]

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(1 Publication)

Mouse Monoclonal NCAM1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing NCAM1 protein.

View Alternative Names

CD56, NCAM, NCAM1, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1

1 Images
Flow Cytometry - FITC Anti-NCAM1 antibody [MEM-188] (AB69763)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-NCAM1 antibody [MEM-188] (AB69763)

ab69763 at 20μl/106 cells staining NCAM in human peripheral blood cells by flow cytometry.

  • Unconjugated

    Anti-NCAM1 antibody [MEM-188]

  • 660 APC

    APC Anti-NCAM1 antibody [MEM-188]

  • 778 PE/Cy7®

    PE/Cy7® Anti-NCAM1 antibody [MEM-188]

  • 675 PerCP

    PerCP Anti-NCAM1 antibody [MEM-188]

  • Biotin

    Biotin Anti-NCAM1 antibody [MEM-188]

  • 578 PE

    PE Anti-NCAM1 antibody [MEM-188]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-188

Isotype

IgG2a

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing NCAM1 protein. The exact immunogen used to generate this antibody is proprietary information.

P13591

Specificity

Reacts with a 180 kDa isoform of NCAM expressed in leukocytes. Reactivity with other isoforms has not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/fitc-mouse-igg2a-x5563-isotype-control-ab91362'>ab91362</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions from >95% pure antibody (SDS PAGE). The reagent is free of unconjugated FITC and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.. (Microbial infection) Acts as a receptor for rabies virus.. (Microbial infection) Acts as a receptor for Zika virus.
See full target information NCAM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental immunology 137:529-37 PubMed15320902

2004

Human macrophages kill human mesangial cells by Fas-L-induced apoptosis when triggered by antibody via CD16.

Applications

Unspecified application

Species

Unspecified reactive species

J J Boyle
View all publications
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