Mouse Monoclonal NCAM1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - FITC Anti-NCAM1 antibody [MY31] (AB218636), expandable thumbnail](https://content.abcam.com/products/images/fitc-ncam1-antibody-my31-ab218636--flow-cytometry-img13532.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - FITC Anti-NCAM1 antibody [MY31] (AB218636)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Cynomolgus monkey | Predicted |
| Rhesus monkey | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µL for 105-108 Cells | Notes in a final volume of approximately 100 μL. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.(Microbial infection) Acts as a receptor for rabies virus.(Microbial infection) Acts as a receptor for Zika virus.
Alternative names
NCAM, NCAM1, NCAM, Neural cell adhesion molecule 1, N-CAM-1, NCAM-1
Recommended products
Mouse Monoclonal NCAM1 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
FITC
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.12% Sodium dihydrogen phosphate, 0.1% Gelatin- Form
Liquid
- Clonality
Monoclonal
- Clone number
MY31
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
- Purification notes
ab218636 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
- Biological function summary
NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.
- Pathways
The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.
- Associated diseases and disorders
NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - FITC Anti-NCAM1 antibody [MY31] (ab218636), expandable thumbnail](https://content.abcam.com/products/images/fitc-ncam1-antibody-my31-ab218636--flow-cytometry-img13532.jpg "Flow Cytometry - FITC Anti-NCAM1 antibody [MY31] (ab218636)")
Flow Cytometry - FITC Anti-NCAM1 antibody [MY31] (ab218636)
Flow cytometric analysis of Human peripheral blood lymphocytes labeling NCAM with APC Anti-Human CD3 and 5 uL (1 ug) ab218636 (right panel) or 1 ug FITC Mouse IgG1 isotype control (left panel).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com